9T5W
Cryo-EM structure of mutant R61H alphaM/beta2 headpiece complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Isoform 2 of Integrin alpha-M | polymer | 763 | 83970.7 | 1 | UniProt (P11215) | Homo sapiens (Human) | CD11 antigen-like family member B,CR-3 alpha chain,Cell surface glycoprotein MAC-1 subunit alpha,Leukocyte adhesion receptor MO1,Neutrophil adherence receptor |
| 2 | B (B) | Integrin beta-2 | polymer | 469 | 52281.1 | 1 | UniProt (P05107) | Homo sapiens (Human) | Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta,Complement receptor C3 subunit beta |
| 3 | C (C) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
| 4 | D, E (D, E) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 2 | In PDB GlyTouCan (G22768VO) | |||
| 5 | F, G (F, G) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 2 | In PDB GlyTouCan (G42666HT) | |||
| 6 | H, I, J, N, O (A, B) | CALCIUM ION | non-polymer | 40.1 | 5 | Chemie (CA) | |||
| 7 | K, L, P (A, B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 3 | Chemie (NAG) | |||
| 8 | M (B) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 9 | Q, R (A, B) | water | water | 18.0 | 7 | Chemie (HOH) |
Sequence modifications
A: 1 - 754 (UniProt: P11215)
B: 1 - 460 (UniProt: P05107)
| PDB | External Database | Details |
|---|---|---|
| His 61 | Arg 77 | engineered mutation |
| Thr 755 | - | expression tag |
| Gly 756 | - | expression tag |
| Gly 757 | - | expression tag |
| Glu 758 | - | expression tag |
| Asn 759 | - | expression tag |
| Leu 760 | - | expression tag |
| Tyr 761 | - | expression tag |
| Phe 762 | - | expression tag |
| Gln 763 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Pro 461 | - | expression tag |
| Ala 462 | - | expression tag |
| Ala 463 | - | expression tag |
| Glu 464 | - | expression tag |
| Asn 465 | - | expression tag |
| Leu 466 | - | expression tag |
| Tyr 467 | - | expression tag |
| Phe 468 | - | expression tag |
| Gln 469 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 136251.8 | |
| Branched | Number of molecules | 5 |
| Total formula weight | 3257.0 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 919.0 | |
| All* | Total formula weight | 140427.7 |
