9T56

Cryo-EM structure of ISCro4-DBL-TBL-tDNA-dDNA synaptic complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	ISCro4 transposase	polymer	341	38131.7	4	UniProt (D2TGM5)	Citrobacter rodentium ICC168
2	E (E)	Target-binding loop (TBL) RNA	polymer	111	35682.1	1	GenBank (282947233)	Citrobacter rodentium ICC168
3	F (F)	Donor-binding loop (DBL) RNA	polymer	79	25278.9	1		Citrobacter rodentium ICC168
4	G (G)	target DNA (tRNA) top strand	polymer	38	11616.5	1		Citrobacter rodentium ICC168
5	H (H)	target DNA (tDNA) bottom strand	polymer	38	11776.6	1		Citrobacter rodentium ICC168
6	I (I)	donor DNA (dDNA) top strand	polymer	44	13562.7	1		Citrobacter rodentium ICC168
7	J (J)	donor DNA (dDNA) bottom strand	polymer	44	13531.7	1		Citrobacter rodentium ICC168
8	K, L (G, I)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B, C, D: 1 - 326 (UniProt: D2TGM5)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag
Ser 327	-	expression tag
Ser 328	-	expression tag
Gly 329	-	expression tag
Ser 330	-	expression tag
Leu 331	-	expression tag
Glu 332	-	expression tag
His 333	-	expression tag
His 334	-	expression tag
His 335	-	expression tag
His 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag

E: 1 - 104 (GenBank: 282947233)

PDB	External Database	Details
G -2	-	expression tag
G -1	-	expression tag
G 0	-	expression tag
U 105	-	expression tag
U 106	-	expression tag
U 107	-	expression tag
U 108	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	263975.4
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	264024.0

*Water molecules are not included.