9T4N

Structure of the Q318A mutant of the SUN4 domain from Saccharomyces cerevisiae

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Probable secreted beta-glucosidase SUN4	polymer	295	31136.7	1	UniProt (P53616)	Saccharomyces cerevisiae S288C	Septation protein SUN4,Soluble cell wall protein 3
2	B (A)	HEXAETHYLENE GLYCOL	non-polymer		282.3	1	Chemie (P6G)
3	C (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	D (A)	water	water		18.0	364	Chemie (HOH)

Sequence modifications

A: 147 - 420 (UniProt: P53616)

PDB	External Database	Details
Met 126	-	initiating methionine
Gly 127	-	expression tag
Ser 128	-	expression tag
Ser 129	-	expression tag
His 130	-	expression tag
His 131	-	expression tag
His 132	-	expression tag
His 133	-	expression tag
His 134	-	expression tag
His 135	-	expression tag
Ser 136	-	expression tag
Ser 137	-	expression tag
Gly 138	-	expression tag
Leu 139	-	expression tag
Val 140	-	expression tag
Pro 141	-	expression tag
Arg 142	-	expression tag
Gly 143	-	expression tag
Ser 144	-	expression tag
His 145	-	expression tag
Asn 146	-	expression tag
Ala 318	Gln 318	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	31136.7
Non-Polymers*	Number of molecules	2
	Total formula weight	317.8
All*	Total formula weight	31454.5

*Water molecules are not included.