9T3X

cryo-EM structure of CPSF160-WDR33-ZC3H18

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cleavage and polyadenylation specificity factor subunit 1	polymer	1443	161074.2	1	UniProt (Q10570) Pfam (PF10433) Pfam (PF23726) Pfam (PF03178)	Homo sapiens (human)
2	B (B)	pre-mRNA 3' end processing protein WDR33	polymer	466	53164.0	1	UniProt (Q9C0J8) Pfam (PF00400)	Homo sapiens (human)	WD repeat-containing protein 33,WD repeat-containing protein of 146 kDa
3	C (C)	Maltose/maltodextrin-binding periplasmic protein,Zinc finger CCCH domain-containing protein 18	polymer	464	50946.8	1	UniProt (P0AEX9) UniProt (Q86VM9) Pfam (PF01547)	Escherichia coli (strain K12)	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Nuclear protein NHN1

Sequence modifications

B: 1 - 413 (UniProt: Q9C0J8)

PDB	External Database	Details
Met -44	-	initiating methionine
Ser -43	-	expression tag
Ala -42	-	expression tag
Trp -41	-	expression tag
Ser -40	-	expression tag
His -39	-	expression tag
Pro -38	-	expression tag
Gln -37	-	expression tag
Phe -36	-	expression tag
Glu -35	-	expression tag
Lys -34	-	expression tag
Gly -33	-	expression tag
Gly -32	-	expression tag
Gly -31	-	expression tag
Ser -30	-	expression tag
Gly -29	-	expression tag
Gly -28	-	expression tag
Gly -27	-	expression tag
Ser -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Ser -23	-	expression tag
Ala -22	-	expression tag
Trp -21	-	expression tag
Ser -20	-	expression tag
His -19	-	expression tag
Pro -18	-	expression tag
Gln -17	-	expression tag
Phe -16	-	expression tag
Glu -15	-	expression tag
Lys -14	-	expression tag
Thr -13	-	expression tag
Ala -12	-	expression tag
Gly -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Asp -2	-	expression tag
Val -1	-	expression tag
Pro 0	-	expression tag
Asp 414	-	expression tag
Tyr 415	-	expression tag
Lys 416	-	expression tag
Asp 417	-	expression tag
Asp 418	-	expression tag
Asp 419	-	expression tag
Asp 420	-	expression tag
Lys 421	-	expression tag

C: -286 - 79 (UniProt: P0AEX9)

PDB	External Database	Details
Met -294	-	initiating methionine
His -293	-	expression tag
His -292	-	expression tag
His -291	-	expression tag
His -290	-	expression tag
His -289	-	expression tag
His -288	-	expression tag
Gly -287	-	expression tag
Asn 80	-	linker
Ser 81	-	linker
Ser 82	-	linker
Ser 83	-	linker
Asn 84	-	linker
Asn 85	-	linker
Asn 86	-	linker
Asn 87	-	linker
Thr 88	-	linker
Ser 89	-	linker
Leu 90	-	linker
Glu 91	-	linker
Val 92	-	linker
Leu 93	-	linker
Phe 94	-	linker
Gln 95	-	linker
Gly 96	-	linker
Pro 97	-	linker
Asp 98	-	linker
Ser 99	-	linker
Met 100	-	linker

C: 101 - 796 (UniProt: Q86VM9)
C: 805 - 830 (UniProt: Q86VM9)

PDB	External Database	Details
Gly 797	-	linker
Ser 798	-	linker
Gly 799	-	linker
Ser 800	-	linker
Gly 801	-	linker
Ser 802	-	linker
Gly 803	-	linker
Ser 804	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	265185.0
All*	Total formula weight	265185.0

*Water molecules are not included.