9T38

Rhs2-CT endonuclease toxin in complex with cognate immunity protein RhsI2 and EF-Tu

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (C, F)	Elongation factor Tu 2	polymer	394	43370.5	2	UniProt (P0CE48) Pfam (PF00009) Pfam (PF03144) Pfam (PF03143)	Escherichia coli	EF-Tu 2,Bacteriophage Q beta RNA-directed RNA polymerase subunit III,P-43
2	B, E (B, E)	Immunity protein RhsI2	polymer	162	18682.3	2	UniProt (A0ABF7SXG6)	Serratia marcescens
3	C, F (A, D)	Rhs-family protein	polymer	162	17797.0	2	UniProt (A0ABC9II69)	Serratia marcescens	Rhs2CT endonuclease toxin
4	G, K (C, F)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
5	H, L (C, F)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
6	I, M (C, F)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	J, N (C, F)	IMIDAZOLE	non-polymer		69.1	2	Chemie (IMD)
8	O, P, Q, R, S... (C, B, A, F, E...)	water	water		18.0	146	Chemie (HOH)

Sequence modifications

A, D: 1290 - 1430 (UniProt: A0ABC9II69)

PDB	External Database	Details
Met 1269	-	initiating methionine
Gly 1270	-	expression tag
Ser 1271	-	expression tag
Ser 1272	-	expression tag
His 1273	-	expression tag
His 1274	-	expression tag
His 1275	-	expression tag
His 1276	-	expression tag
His 1277	-	expression tag
His 1278	-	expression tag
Ser 1279	-	expression tag
Ser 1280	-	expression tag
Gly 1281	-	expression tag
Glu 1282	-	expression tag
Asn 1283	-	expression tag
Leu 1284	-	expression tag
Tyr 1285	-	expression tag
Phe 1286	-	expression tag
Gln 1287	-	expression tag
Gly 1288	-	expression tag
Gly 1289	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	159699.6
Non-Polymers*	Number of molecules	8
	Total formula weight	1197.3
All*	Total formula weight	160896.9

*Water molecules are not included.