9T0V
Crystal structure of H416C NikA mutant from Escherichia coli in complex with Fe(III)-EDTA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Nickel-binding periplasmic protein | polymer | 502 | 56325.7 | 2 | UniProt (P33590) | Escherichia coli K-12 | |
| 2 | C, J (A, B) | FE (III) ION | non-polymer | 55.8 | 2 | Chemie (FE) PubChem (29936) | |||
| 3 | D, K (A, B) | {[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC ACID | non-polymer | 292.2 | 2 | Chemie (EDT) PubChem (6049) | |||
| 4 | E (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) PubChem (312) | |||
| 5 | F, L, M (A, B) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) PubChem (753) | |||
| 6 | G, H (A) | ACETATE ION | non-polymer | 59.0 | 2 | Chemie (ACT) PubChem (175) | |||
| 7 | I (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 8 | N, O (A, B) | water | water | 18.0 | 178 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 502 (UniProt: P33590)
| PDB | External Database | Details |
|---|---|---|
| Cys 416 | His 438 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 112651.5 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 1222.1 | |
| All* | Total formula weight | 113873.5 |
