9T0A

X-ray structure of the complex between the catalytic domain of CDC25B C473S and 3-O-methylfluorescein phosphate (3-OMFP)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	M-phase inducer phosphatase 2	polymer	197	22995.3	1	UniProt (P30305) Pfam (PF00581)	Homo sapiens (human)	Dual specificity phosphatase Cdc25B
2	B (A)	[(1~{R})-6'-methoxy-3-oxidanylidene-spiro[2-benzofuran-1,9'-xanthene]-3'-yl] dihydrogen phosphate	non-polymer		426.3	1	Chemie (A1JSQ)
3	C (A)	[(1~{S})-6'-methoxy-3-oxidanylidene-spiro[2-benzofuran-1,9'-xanthene]-3'-yl] dihydrogen phosphate	non-polymer		426.3	1	Chemie (A1JSP)
4	D, E, F (A)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
5	G (A)	water	water		18.0	119	Chemie (HOH)

Sequence modifications

A: 377 - 550 (UniProt: P30305)

PDB	External Database	Details
Met 354	-	initiating methionine
Gly 355	-	expression tag
Ser 356	-	expression tag
Ser 357	-	expression tag
His 358	-	expression tag
His 359	-	expression tag
His 360	-	expression tag
His 361	-	expression tag
His 362	-	expression tag
His 363	-	expression tag
Ser 364	-	expression tag
Ser 365	-	expression tag
Gly 366	-	expression tag
Leu 367	-	expression tag
Val 368	-	expression tag
Pro 369	-	expression tag
Arg 370	-	expression tag
Gly 371	-	expression tag
Ser 372	-	expression tag
His 373	-	expression tag
Met 374	-	expression tag
Ala 375	-	expression tag
Ser 376	-	expression tag
Ser 473	Cys 487	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	22995.3
Non-Polymers*	Number of molecules	5
	Total formula weight	959.0
All*	Total formula weight	23954.2

*Water molecules are not included.