9SV3

Local refinement of EloB/EloC/VHL/CV2a/14-3-3zeta/ERa from pose 1

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Elongin-C	polymer	97	10974.6	1	UniProt (Q15369)	Homo sapiens (human)	EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1
2	B (C)	Elongin-B	polymer	104	11748.4	1	UniProt (Q15370)	Homo sapiens (human)	EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2
3	C, D (G, H)	Estrogen receptor	polymer	401	45511.2	2	UniProt (P03372)	Homo sapiens (human)	ER,ER-alpha,Estradiol receptor,Nuclear receptor subfamily 3 group A member 1
4	E, F (I, D)	14-3-3 protein zeta/delta	polymer	253	28819.2	2	UniProt (P63104)	Homo sapiens (human)	Protein kinase C inhibitor protein 1,KCIP-1
5	G (E)	von Hippel-Lindau disease tumor suppressor	polymer	162	18702.3	1	UniProt (P40337)	Homo sapiens (human)	Protein G7,pVHL
6	H (D)	~{N}'-[(2~{S})-3,3-dimethyl-1-[(2~{S},4~{R})-2-[[4-(4-methyl-1,3-thiazol-5-yl)phenyl]methylcarbamoyl]-4-oxidanyl-pyrrolidin-1-yl]-1-oxidanylidene-butan-2-yl]-~{N}-[(2~{S})-2-[(1~{E},3~{R},4~{S},8~{R},9~{R},10~{R},11~{S},14~{S})-14-(methoxymethyl)-3,10-dimethyl-8-[(2~{S},3~{R},4~{S},5~{S},6~{R})-6-(2-methylbut-3-en-2-yloxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-4,9-bis(oxidanyl)-6-tricyclo[9.3.0.0^{3,7}]tetradeca-1,6-dienyl]propyl]tridecanediamide	non-polymer		1234.6	1	Chemie (A1JQT)

Sequence modifications

B: 17 - 112 (UniProt: Q15369)

PDB	External Database	Details
Met 16	-	initiating methionine

G, H: 302 - 595 (UniProt: P03372)

PDB	External Database	Details
Met 195	-	initiating methionine
Gly 196	-	expression tag
His 197	-	expression tag
His 198	-	expression tag
His 199	-	expression tag
His 200	-	expression tag
His 201	-	expression tag
His 202	-	expression tag
Gly 203	-	expression tag
Gly 204	-	expression tag
Ser 205	-	expression tag
Asp 206	-	expression tag
Ser 207	-	expression tag
Glu 208	-	expression tag
Val 209	-	expression tag
Asn 210	-	expression tag
Gln 211	-	expression tag
Glu 212	-	expression tag
Ala 213	-	expression tag
Lys 214	-	expression tag
Pro 215	-	expression tag
Glu 216	-	expression tag
Val 217	-	expression tag
Lys 218	-	expression tag
Pro 219	-	expression tag
Glu 220	-	expression tag
Val 221	-	expression tag
Lys 222	-	expression tag
Pro 223	-	expression tag
Glu 224	-	expression tag
Thr 225	-	expression tag
His 226	-	expression tag
Ile 227	-	expression tag
Asn 228	-	expression tag
Leu 229	-	expression tag
Lys 230	-	expression tag
Val 231	-	expression tag
Ser 232	-	expression tag
Asp 233	-	expression tag
Gly 234	-	expression tag
Ser 235	-	expression tag
Ser 236	-	expression tag
Glu 237	-	expression tag
Ile 238	-	expression tag
Phe 239	-	expression tag
Phe 240	-	expression tag
Lys 241	-	expression tag
Ile 242	-	expression tag
Lys 243	-	expression tag
Lys 244	-	expression tag
Thr 245	-	expression tag
Thr 246	-	expression tag
Pro 247	-	expression tag
Leu 248	-	expression tag
Arg 249	-	expression tag
Arg 250	-	expression tag
Leu 251	-	expression tag
Met 252	-	expression tag
Glu 253	-	expression tag
Ala 254	-	expression tag
Phe 255	-	expression tag
Ala 256	-	expression tag
Lys 257	-	expression tag
Arg 258	-	expression tag
Gln 259	-	expression tag
Gly 260	-	expression tag
Lys 261	-	expression tag
Glu 262	-	expression tag
Met 263	-	expression tag
Asp 264	-	expression tag
Ser 265	-	expression tag
Leu 266	-	expression tag
Arg 267	-	expression tag
Phe 268	-	expression tag
Leu 269	-	expression tag
Tyr 270	-	expression tag
Asp 271	-	expression tag
Gly 272	-	expression tag
Ile 273	-	expression tag
Arg 274	-	expression tag
Ile 275	-	expression tag
Gln 276	-	expression tag
Ala 277	-	expression tag
Asp 278	-	expression tag
Gln 279	-	expression tag
Thr 280	-	expression tag
Pro 281	-	expression tag
Glu 282	-	expression tag
Asp 283	-	expression tag
Leu 284	-	expression tag
Asp 285	-	expression tag
Met 286	-	expression tag
Glu 287	-	expression tag
Asp 288	-	expression tag
Asn 289	-	expression tag
Asp 290	-	expression tag
Ile 291	-	expression tag
Ile 292	-	expression tag
Glu 293	-	expression tag
Ala 294	-	expression tag
His 295	-	expression tag
Arg 296	-	expression tag
Glu 297	-	expression tag
Gln 298	-	expression tag
Ile 299	-	expression tag
Gly 300	-	expression tag
Gly 301	-	expression tag
Ala 305	Ser 305	engineered mutation
Arg 591	Phe 591	engineered mutation
Arg 592	Pro 592	engineered mutation

I, D: 1 - 245 (UniProt: P63104)

PDB	External Database	Details
Trp 246	-	expression tag
Ser 247	-	expression tag
His 248	-	expression tag
Pro 249	-	expression tag
Gln 250	-	expression tag
Phe 251	-	expression tag
Glu 252	-	expression tag
Lys 253	-	expression tag

E: 54 - 213 (UniProt: P40337)

PDB	External Database	Details
Gly 52	-	expression tag
Ser 53	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	190086.2
Non-Polymers*	Number of molecules	1
	Total formula weight	1234.6
All*	Total formula weight	191320.8

*Water molecules are not included.