9SRW

Cryo-EM structure of the Mlc tetramer in complex with the anti-repressor MtfA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (E, F)	Mlc titration factor A	polymer	287	33027.0	2	UniProt (P76346)	Escherichia coli	Mlc-binding protein,Probable zinc metallopeptidase MtfA
2	B, C, E, F (A, B, C, D)	DNA-binding transcriptional repressor Mlc	polymer	455	49903.0	4	UniProt (P50456)	Escherichia coli	Making large colonies protein,Membrane linked control
3	G, H, I, J, K... (E, A, B, F, C...)	ZINC ION	non-polymer		65.4	6	Chemie (ZN) PubChem (32051)

Sequence modifications

E, F: 1 - 265 (UniProt: P76346)

PDB	External Database	Details
Leu 266	-	expression tag
Glu 267	-	expression tag
Leu 268	-	expression tag
Glu 269	-	expression tag
Val 270	-	expression tag
Leu 271	-	expression tag
Phe 272	-	expression tag
Gln 273	-	expression tag
Gly 274	-	expression tag
Pro 275	-	expression tag
Val 276	-	expression tag
Asp 277	-	expression tag
His 278	-	expression tag
His 279	-	expression tag
His 280	-	expression tag
His 281	-	expression tag
His 282	-	expression tag
His 283	-	expression tag
His 284	-	expression tag
His 285	-	expression tag
His 286	-	expression tag
His 287	-	expression tag

A, B, C, D: 1 - 406 (UniProt: P50456)

PDB	External Database	Details
Met -46	-	initiating methionine
Gly -45	-	expression tag
Gly -44	-	expression tag
Ser -43	-	expression tag
His -42	-	expression tag
His -41	-	expression tag
His -40	-	expression tag
His -39	-	expression tag
His -38	-	expression tag
His -37	-	expression tag
Gly -36	-	expression tag
Met -35	-	expression tag
Ala -34	-	expression tag
Ser -33	-	expression tag
Met -32	-	expression tag
Thr -31	-	expression tag
Gly -30	-	expression tag
Gly -29	-	expression tag
Gln -28	-	expression tag
Gln -27	-	expression tag
Met -26	-	expression tag
Gly -25	-	expression tag
Arg -24	-	expression tag
Asp -23	-	expression tag
Leu -22	-	expression tag
Tyr -21	-	expression tag
Asp -20	-	expression tag
Asp -19	-	expression tag
Asp -18	-	expression tag
Asp -17	-	expression tag
Lys -16	-	expression tag
Asp -15	-	expression tag
Arg -14	-	expression tag
Trp -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Glu -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Val -7	-	expression tag
Leu -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
Lys -1	-	expression tag
Leu 0	-	expression tag
Leu 407	-	expression tag
Glu 408	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	265666.0
Non-Polymers*	Number of molecules	6
	Total formula weight	392.5
All*	Total formula weight	266058.4

*Water molecules are not included.