9SQ6

Cryo-EM structure of the DDB1deltaB-CRBN-ALV2 complex bound to HELIOS

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA damage-binding protein 1	polymer	864	96425.6	1	UniProt (Q16531)	Homo sapiens (human)	DDB p127 subunit,DNA damage-binding protein a,DDBa,Damage-specific DNA-binding protein 1,HBV X-associated protein 1,XAP-1,UV-damaged DNA-binding factor,UV-damaged DNA-binding protein 1,UV-DDB 1,XPE-binding factor,XPE-BF,Xeroderma pigmentosum group E-complementing protein,XPCe
2	B (B)	Protein cereblon	polymer	466	53290.5	1	UniProt (Q96SW2)	Homo sapiens (human)
3	C (C)	Zinc finger protein Helios	polymer	124	13920.1	1	UniProt (Q9UKS7)	Homo sapiens (human)	Ikaros family zinc finger protein 2
4	D, F, G (B, C)	ZINC ION	non-polymer		65.4	3	Chemie (ZN) PubChem (32051)
5	E (B)	~{N}-[2-[3-[[1-[(3~{S})-2,6-bis(oxidanylidene)piperidin-3-yl]-2,5-bis(oxidanylidene)pyrrol-3-yl]amino]phenyl]propan-2-yl]-2-[(3-chloranyl-4-methyl-phenyl)amino]ethanamide	non-polymer		538.0	1	Chemie (A1JPR)

Sequence modifications

A: 1 - 699 (UniProt: Q16531)

PDB	External Database	Details
Met -27	-	initiating methionine
Gly -26	-	expression tag
Ser -25	-	expression tag
Ser -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
Ser -17	-	expression tag
Ala -16	-	expression tag
Ala -15	-	expression tag
His -14	-	expression tag
Ile -13	-	expression tag
Val -12	-	expression tag
Met -11	-	expression tag
Val -10	-	expression tag
Asp -9	-	expression tag
Ala -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Pro -5	-	expression tag
Thr -4	-	expression tag
Lys -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

A: 706 - 1140 (UniProt: Q16531)

PDB	External Database	Details
Gly 700	-	linker
Asn 701	-	linker
Gly 702	-	linker
Asn 703	-	linker
Ser 704	-	linker
Gly 705	-	linker

B: 1 - 442 (UniProt: Q96SW2)

PDB	External Database	Details
Met -23	-	initiating methionine
Gly -22	-	expression tag
Ser -21	-	expression tag
Ser -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Ser -13	-	expression tag
Ala -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

C: 94 - 194 (UniProt: Q9UKS7)

PDB	External Database	Details
Met 71	-	initiating methionine
Asp 72	-	expression tag
Trp 73	-	expression tag
Ser 74	-	expression tag
His 75	-	expression tag
Pro 76	-	expression tag
Gln 77	-	expression tag
Phe 78	-	expression tag
Glu 79	-	expression tag
Lys 80	-	expression tag
Ser 81	-	expression tag
Ala 82	-	expression tag
Val 83	-	expression tag
Asp 84	-	expression tag
Glu 85	-	expression tag
Asn 86	-	expression tag
Leu 87	-	expression tag
Tyr 88	-	expression tag
Phe 89	-	expression tag
Gln 90	-	expression tag
Gly 91	-	expression tag
Gly 92	-	expression tag
Gly 93	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	163636.2
Non-Polymers*	Number of molecules	4
	Total formula weight	734.2
All*	Total formula weight	164370.4

*Water molecules are not included.