9SOI

CTLH-CRA domains of Maea-Twa1 mutant (A125G, Q126R, T127del, Q128E, A130Q, M143L, E144Q, L147F, A148S, F152Y and F160V) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Glucose-induced degradation protein 8 homolog	polymer	133	15681.6	2	UniProt (Q9D7M1)	Mus musculus (house mouse)	Two hybrid-associated protein 1 with RanBPM,Twa1
2	B, D (B, D)	E3 ubiquitin-protein transferase MAEA	polymer	126	15116.4	2	UniProt (Q4VC33)	Mus musculus (house mouse)	Erythroblast macrophage protein,Macrophage erythroblast attacher

Sequence modifications

A, C: 1 - 133 (UniProt: Q9D7M1)

PDB	External Database	Details
Gly 69	Ala 125	engineered mutation
Arg 70	Gln 126	engineered mutation
-	Thr 127	deletion
Glu 71	Gln 128	engineered mutation
Gln 73	Ala 130	engineered mutation
Leu 86	Met 143	engineered mutation
Gln 87	Glu 144	engineered mutation
Phe 90	Leu 147	engineered mutation
Ser 91	Ala 148	engineered mutation
Tyr 95	Phe 152	engineered mutation
Val 103	Phe 160	engineered mutation

B, D: 1 - 124 (UniProt: Q4VC33)

PDB	External Database	Details
Gly -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	61595.9
All*	Total formula weight	61595.9

*Water molecules are not included.