9SMX

CM1-activated gTuRC in complex with nascent alpha-E254D mutant microtubules

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, BA, EB, H, LA... (3, B, D, F, H...)	Gamma-tubulin complex component 3	polymer	907	103710.1	6	UniProt (Q96CW5)	Homo sapiens (human)	hGCP3,Gamma-ring complex protein 104 kDa,hGrip104,Spindle pole body protein Spc98 homolog,hSpc98
2	B, D, FB (4, 6, Y)	Mitotic-spindle organizing protein 1	polymer	82	8485.7	3	UniProt (Q08AG7)	Homo sapiens (human)	Mitotic-spindle organizing protein associated with a ring of gamma-tubulin 1
3	C, XA (5, L)	Gamma-tubulin complex component 6	polymer	1819	200733.6	2	UniProt (Q96RT7)	Homo sapiens (human)	GCP-6
4	E (7)	Actin, cytoplasmic 2, N-terminally processed	polymer	374	41723.5	1	UniProt (P63261)	Homo sapiens (human)
5	AB, EA, F, JA, K... (A, C, CN, E, EN...)	Gamma-tubulin complex component 2	polymer	902	102667.0	8	UniProt (Q9BSJ2)	Homo sapiens (human)	hGCP2,Gamma-ring complex protein 103 kDa,hGrip103,Spindle pole body protein Spc97 homolog,hSpc97
6	AA, BB, DB, G, HA... (AC, CC, Cc, EC, Ec...)	Ubiquitin-like protein SMT3,CDK5 regulatory subunit-associated protein 2,Microtubule-associated protein RP/EB family member 1	polymer	229	26766.0	9	UniProt (Q12306) UniProt (Q96SN8) UniProt (Q15691)	Saccharomyces cerevisiae S288C	CDK5 activator-binding protein C48,Centrosome-associated protein 215,APC-binding protein EB1,End-binding protein 1,EB1
7	CA, FA, I, L, MA... (BA, CA, DA, EA, FA...)	Isoform 1 of Tubulin alpha-1B chain	polymer	451	50190.4	11	UniProt (P68363)	Homo sapiens (human)	Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain
8	DA, GA, J, M, NA... (BB, CB, DB, EB, FB...)	Tubulin beta-3 chain	polymer	451	50906.7	11	UniProt (Q13509)	Homo sapiens (human)	Tubulin beta-4 chain,Tubulin beta-III
9	CB, IA, O, Y (CM, EM, GM, MM)	Mitotic-spindle organizing protein 2A	polymer	158	16240.6	4	UniProt (Q6P582)	Homo sapiens (human)	Mitotic-spindle organizing protein associated with a ring of gamma-tubulin 2A
10	OA, UA (I, K)	Gamma-tubulin complex component 4	polymer	667	76180.0	2	UniProt (Q9UGJ1)	Homo sapiens (human)	hGCP4,Gamma-ring complex protein 76 kDa,hGrip76
11	GB, RA (J, Z)	Gamma-tubulin complex component 5	polymer	1024	118467.5	2	UniProt (Q96RT8)	Homo sapiens (human)	GCP-5
12	HB, IB, JB, KB, LB... (a, b, c, d, e...)	Tubulin gamma-1 chain	polymer	451	51227.8	14	UniProt (P23258)	Homo sapiens (human)	Gamma-1-tubulin,Gamma-tubulin complex component 1,GCP-1
13	AC, BC, CC, DC, EC... (GA, HA, IA, JA, KA...)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	11	Chemie (GTP)

Sequence modifications

7: 2 - 375 (UniProt: P63261)

PDB	External Database	Details
Asp 2	Glu 2	conflict
Val 5	Ile 5	conflict
Val 10	Ile 10	conflict
Leu 153	Met 153	conflict
Ser 160	Thr 160	conflict
Glu 204	Ala 204	conflict
Lys 243	Pro 243	conflict
Ala 271	Ser 271	conflict
Tyr 279	Phe 279	conflict

AC, CC, Cc, EC, Ec, GC, Gc, MC, Mc: -57 - 40 (UniProt: Q12306)

PDB	External Database	Details
Met -68	-	initiating methionine
Gly -67	-	expression tag
Ser -66	-	expression tag
Ser -65	-	expression tag
His -64	-	expression tag
His -63	-	expression tag
His -62	-	expression tag
His -61	-	expression tag
His -60	-	expression tag
His -59	-	expression tag
Gly -58	-	expression tag
Trp 41	-	linker
Ser 42	-	linker
His 43	-	linker
Pro 44	-	linker
Gln 45	-	linker
Phe 46	-	linker
Glu 47	-	linker
Lys 48	-	linker
Ser 49	-	linker
Ala 50	-	linker

AC, CC, Cc, EC, Ec, GC, Gc, MC, Mc: 51 - 94 (UniProt: Q96SN8)
AC, CC, Cc, EC, Ec, GC, Gc, MC, Mc: 95 - 160 (UniProt: Q15691)
BA, CA, DA, EA, FA, GA, HA, IA, JA, KA, LA: 1 - 451 (UniProt: P68363)

PDB	External Database	Details
Asp 254	Glu 254	engineered mutation

BB, CB, DB, EB, FB, GB, HB, IB, JB, KB, LB: 1 - 500 (UniProt: Q13509)

PDB	External Database	Details
Gdp 501	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	73
	Total formula weight	4436652.3
Non-Polymers*	Number of molecules	11
	Total formula weight	5755.0
All*	Total formula weight	4442407.3

*Water molecules are not included.