9SMP

BRCA1-A complex bound to K63-polyUbATA - open form double State P

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (A, F)	BRCA1-A complex subunit Abraxas 1	polymer	409	46731.8	2	UniProt (Q6UWZ7) Pfam (PF21125)	Homo sapiens (human)	Coiled-coil domain-containing protein 98,Protein FAM175A
2	B, G (B, G)	Lys-63-specific deubiquitinase BRCC36	polymer	316	36119.9	2	UniProt (P46736) Pfam (PF01398) Pfam (PF18110)	Homo sapiens (human)	BRCA1-A complex subunit BRCC36,BRCA1/BRCA2-containing complex subunit 3,BRCA1/BRCA2-containing complex subunit 36,BRISC complex subunit BRCC36
3	C, H (C, H)	BRISC and BRCA1-A complex member 2	polymer	383	43593.5	2	UniProt (Q9NXR7) Pfam (PF06113)	Homo sapiens (human)	BRCA1-A complex subunit BRE,BRCA1/BRCA2-containing complex subunit 45,Brain and reproductive organ-expressed protein
4	D, I (D, I)	BRISC and BRCA1-A complex member 1	polymer	349	38796.4	2	UniProt (Q9NWV8)	Homo sapiens (human)	Mediator of RAP80 interactions and targeting subunit of 40 kDa,New component of the BRCA1-A complex
5	E, J (E, J)	BRCA1-A complex subunit RAP80	polymer	171	19158.2	2	UniProt (Q96RL1)	Homo sapiens (human)	Receptor-associated protein 80,Retinoid X receptor-interacting protein 110,Ubiquitin interaction motif-containing protein 1
6	K, M (K, M)	Polyubiquitin-C	polymer	76	8547.8	2	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens
7	L (L)	Polyubiquitin-C	polymer	76	8636.9	1	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens
8	N (N)	Polyubiquitin-C	polymer	76	8636.9	1	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens
9	O, P, Q, R (S, T, U, V)	Ubiquitin	polymer	76	8576.8	4	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens	Polyubiquitin-C
10	S, T (B, G)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)

Sequence modifications

D, I: 1 - 329 (UniProt: Q9NWV8)

PDB	External Database	Details
Met -19	-	initiating methionine
Ala -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ala -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag

E, J: 265 - 413 (UniProt: Q96RL1)

PDB	External Database	Details
Met 243	-	initiating methionine
Ala 244	-	expression tag
Ser 245	-	expression tag
Trp 246	-	expression tag
Ser 247	-	expression tag
His 248	-	expression tag
Pro 249	-	expression tag
Gln 250	-	expression tag
Phe 251	-	expression tag
Glu 252	-	expression tag
Lys 253	-	expression tag
Gly 254	-	expression tag
Ala 255	-	expression tag
Leu 256	-	expression tag
Glu 257	-	expression tag
Val 258	-	expression tag
Leu 259	-	expression tag
Phe 260	-	expression tag
Gln 261	-	expression tag
Gly 262	-	expression tag
Pro 263	-	expression tag
Gly 264	-	expression tag

K, M: 1 - 76 (UniProt: P0CG48)

PDB	External Database	Details
Dab 63	Lys 63	engineered mutation

L: 1 - 76 (UniProt: P0CG48)

PDB	External Database	Details
A1jpl 76	Gly 76	engineered mutation

N: 1 - 76 (UniProt: P0CG48)

PDB	External Database	Details
A1jpt 76	Gly 76	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	18
	Total formula weight	437476.1
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	437607.0

*Water molecules are not included.