9SL1

GABA-A a4b3d receptor in complex with GABA, Nb24 and Mb30

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Gamma-aminobutyric acid receptor subunit alpha-4	polymer	364	41783.3	2	UniProt (P48169)	Homo sapiens (human)	GABA(A) receptor subunit alpha-4,GABAAR subunit alpha-4
2	B, D (B, D)	Gamma-aminobutyric acid receptor subunit beta-3,Soluble cytochrome b562	polymer	451	51615.1	2	UniProt (P28472) UniProt (P0ABE7)	Homo sapiens (human)	GABA(A) receptor subunit beta-3,GABAAR subunit beta-3,Cytochrome b-562
3	E (E)	GABA-A receptor SBP-delta-glvi,Gamma-aminobutyric acid receptor subunit delta,Gamma-aminobutyric acid receptor subunit delta	polymer	430	48234.0	1	UniProt (O14764)	Homo sapiens	GABA(A) receptor subunit delta,GABAAR subunit delta
4	F, H (F, H)	Megabody Mb30	polymer	138	15386.9	2		Lama glama
5	G, I (G, I)	Nanobody Nb24	polymer	133	14689.1	2		Lama glama
6	J (Q)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G53081KU)
7	K (R)	beta-D-mannopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB
8	L, M, Q, R, T... (A, B, C, D, E)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	7	Chemie (NAG) PubChem (24139)
9	N (A)	1,2-DIPALMITOYL-SN-GLYCERO-3-PHOSPHATE	non-polymer		647.9	1	Chemie (PX6)
10	O, S, W, Y (A, B, D, E)	DECANE	non-polymer		142.3	4	Chemie (D10) PubChem (15600)
11	P, U (B, D)	GAMMA-AMINO-BUTANOIC ACID	non-polymer		103.1	2	Chemie (ABU) PubChem (119)
12	AA, Z (C, A)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

A, C: 11 - 310 (UniProt: P48169)

PDB	External Database	Details
Gly 1	-	expression tag
Asn 2	-	expression tag
Ser 3	-	expression tag
Lys 4	-	expression tag
Asp 5	-	expression tag
Glu 6	-	expression tag
Lys 7	-	expression tag
Leu 8	-	expression tag
Cys 9	-	expression tag
Pro 10	-	expression tag
Thr 120	Met 155	engineered mutation

A, C: 390 - 427 (UniProt: P48169)

PDB	External Database	Details
Ser 311	-	linker
Gln 312	-	linker
Pro 313	-	linker
Ala 314	-	linker
Arg 315	-	linker
Ala 316	-	linker
Ala 317	-	linker
Gly 428	-	expression tag
Thr 429	-	expression tag
Lys 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag

B, D: 1 - 307 (UniProt: P28472)
B, D: 307 - 311 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 307	Met 29	engineered mutation
Ile 311	His 124	engineered mutation
Leu 311	Arg 128	engineered mutation
Thr 417	-	linker
Gly 418	-	linker
Arg 419	-	linker
Ala 420	-	linker
Ala 421	-	linker

B, D: 422 - 448 (UniProt: P28472)

PDB	External Database	Details
Ser 307	-	linker
Gln 307	-	linker
Pro 307	-	linker
Ala 307	-	linker
Gly 307	-	linker
Thr 307	-	linker

E: -83 - -55 (PDB: 9SL1)

PDB	External Database	Details
Asp -16	-	linker
Tyr -15	-	linker
Asp -14	-	linker
Ile -13	-	linker
Pro -12	-	linker
Thr -11	-	linker
Thr -10	-	linker
Glu -9	-	linker
Asn -8	-	linker
Leu -7	-	linker
Tyr -6	-	linker
Phe -5	-	linker
Gln -4	-	linker
Gly -3	-	linker
Thr -2	-	linker
Gly -1	-	linker

E: -54 - -17 (PDB: 9SL1)
E: 0 - 312 (UniProt: O14764)
E: 392 - 418 (UniProt: O14764)

PDB	External Database	Details
Ser 313	-	linker
Gln 314	-	linker
Pro 315	-	linker
Ala 316	-	linker
Arg 317	-	linker
Ala 318	-	linker
Ala 319	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	295182.9
Branched	Number of molecules	2
	Total formula weight	1173.1
Non-Polymers*	Number of molecules	14
	Total formula weight	2971.7
All*	Total formula weight	299327.7

*Water molecules are not included.