9SJF

Crystal structure of GHdex dextranase (BT3087), E360A catalytic mutant with bound IMO3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cycloisomaltooligosaccharide glucanotransferase	polymer	576	64950.5	2	UniProt (Q8A368)	Bacteroides thetaiotaomicron VPI-5482
2	C, D (C, D)	alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose	branched		504.4	2	In PDB
3	E, F, V, W, X... (A, B)	CHLORIDE ION	non-polymer		35.5	6	Chemie (CL)
4	AA, BA, CA, DA, EA... (B, A)	SULFATE ION	non-polymer		96.1	32	Chemie (SO4)
5	QA, RA (A, B)	water	water		18.0	466	Chemie (HOH)

Sequence modifications

A, B: 26 - 592 (UniProt: Q8A368)

PDB	External Database	Details
Met 25	-	initiating methionine
Ala 360	Glu 360	engineered mutation
Leu 593	-	expression tag
Glu 594	-	expression tag
His 595	-	expression tag
His 596	-	expression tag
His 597	-	expression tag
His 598	-	expression tag
His 599	-	expression tag
His 600	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	129900.9
Branched	Number of molecules	2
	Total formula weight	1008.9
Non-Polymers*	Number of molecules	38
	Total formula weight	3286.7
All*	Total formula weight	134196.6

*Water molecules are not included.