9SJE
Cryo-EM structure of the open-closed dextran utilisome (BT3087-BT3090), with GHdex D297A E360A catalytic inactivation, with bound IMO4, IMO6, IMO7, and IMO8
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | SusC homolog | polymer | 999 | 110591.2 | 2 | UniProt (Q8A365) | Bacteroides thetaiotaomicron VPI-5482 | |
| 2 | B, D (B, D) | SusD homolog | polymer | 506 | 56873.9 | 2 | UniProt (Q8A366) | Bacteroides thetaiotaomicron VPI-5482 | |
| 3 | E, F (E, F) | BT3088 (SGBPdex) | polymer | 504 | 56086.4 | 2 | UniProt (Q8A367) | Bacteroides thetaiotaomicron VPI-5482 | |
| 4 | G, H (G, H) | Cycloisomaltooligosaccharide glucanotransferase | polymer | 592 | 66515.4 | 2 | UniProt (Q8A368) | Bacteroides thetaiotaomicron VPI-5482 | |
| 5 | I, J (I, J) | alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose | branched | 666.6 | 2 | In PDB | |||
| 6 | K (K) | alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose | branched | 1315.1 | 1 | In PDB | |||
| 7 | L, N (L, N) | alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose | branched | 990.9 | 2 | In PDB | |||
| 8 | M (M) | alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose | branched | 1153.0 | 1 | In PDB |
Sequence modifications
B, D: 1 - 496 (UniProt: Q8A366)
G, H: 1 - 592 (UniProt: Q8A368)
| PDB | External Database | Details |
|---|---|---|
| Ala 497 | - | expression tag |
| Ala 498 | - | expression tag |
| Ala 499 | - | expression tag |
| Ala 500 | - | expression tag |
| His 501 | - | expression tag |
| His 502 | - | expression tag |
| His 503 | - | expression tag |
| His 504 | - | expression tag |
| His 505 | - | expression tag |
| His 506 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 297 | Asp 297 | engineered mutation |
| Ala 360 | Glu 360 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 580133.7 | |
| Branched | Number of molecules | 6 |
| Total formula weight | 5783.0 | |
| All* | Total formula weight | 585916.7 |
