9SIU

Type I-F_HNH variant Cascade bound to dsDNA, HNH domain in outwards position

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cas8f fusion with HNH	polymer	384	43706.3	1	UniProt (A0AAX7FM29)	Selenomonas sp.
2	B (B)	Cas5f	polymer	255	28703.1	1	UniProt (A0AAX7FM22)	Selenomonas sp.
3	C (I)	Cas6f	polymer	181	20735.9	1	UniProt (A0AAX7FM27)	Selenomonas sp.
4	D (L)	NTS	polymer	12	3753.5	1		Selenomonas sp.
5	E, F, G, H, I... (D, E, F, C, G...)	Cas7f	polymer	335	38700.2	6	UniProt (A0AAX7FM28)	Selenomonas sp.
6	K (K)	TS	polymer	46	14094.0	1		Selenomonas sp.
7	L (J)	crRNA	polymer	60	19440.6	1		Selenomonas sp.

Sequence modifications

A: 2 - 344 (UniProt: A0AAX7FM29)

PDB	External Database	Details
Met -39	-	initiating methionine
Leu -38	-	expression tag
Arg -37	-	expression tag
Ser -36	-	expression tag
Lys -35	-	expression tag
His -34	-	expression tag
His -33	-	expression tag
His -32	-	expression tag
His -31	-	expression tag
Ser -30	-	expression tag
Gly -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
Thr -25	-	expression tag
Gly -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
Ser -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Thr -13	-	expression tag
Gly -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	362634.5
All*	Total formula weight	362634.5

*Water molecules are not included.