9SHT

Cryo-EM structure of human fibrillar procollagen type I C-propeptide heterotrimer complexed with PCPE-1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Collagen alpha-1(I) chain	polymer	383	40508.7	2	UniProt (P02452) Pfam (PF01391) Pfam (PF01410)	Homo sapiens (human)	Alpha-1 type I collagen
2	C (C)	Collagen alpha-2(I) chain	polymer	400	42339.8	1	UniProt (P08123) Pfam (PF01391) Pfam (PF01410)	Homo sapiens (human)	Alpha-2 type I collagen
3	D (D)	Procollagen C-endopeptidase enhancer 1	polymer	433	46758.6	1	UniProt (Q15113) Pfam (PF00431) Pfam (PF01759)	Homo sapiens (human)	Procollagen COOH-terminal proteinase enhancer 1,PCPE-1,Procollagen C-proteinase enhancer 1,Type 1 procollagen C-proteinase enhancer protein,Type I procollagen COOH-terminal proteinase enhancer
4	E, F, G, H, I (A, B, C, D)	CALCIUM ION	non-polymer		40.1	5	Chemie (CA) PubChem (271)

Sequence modifications

A, B: -124 - 246 (UniProt: P02452)

PDB	External Database	Details
Glu -136	-	expression tag
Thr -135	-	expression tag
Gly -134	-	expression tag
Gly -133	-	expression tag
Pro -132	-	expression tag
Pro -131	-	expression tag
Gly -130	-	expression tag
Pro -129	-	expression tag
Pro -128	-	expression tag
Gly -127	-	expression tag
Pro -126	-	expression tag
Pro -125	-	expression tag
Gln 147	Asn 1365	engineered mutation
Ser 216	Thr 1434	conflict

C: -110 - 247 (UniProt: P08123)

PDB	External Database	Details
Glu -152	-	expression tag
Thr -151	-	expression tag
Gly -150	-	expression tag
Trp -149	-	expression tag
Ser -148	-	expression tag
His -147	-	expression tag
Pro -146	-	expression tag
Gln -145	-	expression tag
Phe -144	-	expression tag
Glu -143	-	expression tag
Lys -142	-	expression tag
Gly -141	-	expression tag
Gly -140	-	expression tag
Gly -139	-	expression tag
Ser -138	-	expression tag
Gly -137	-	expression tag
Gly -136	-	expression tag
Gly -135	-	expression tag
Ser -134	-	expression tag
Gly -133	-	expression tag
Gly -132	-	expression tag
Ser -131	-	expression tag
Ala -130	-	expression tag
Trp -129	-	expression tag
Ser -128	-	expression tag
His -127	-	expression tag
Pro -126	-	expression tag
Gln -125	-	expression tag
Phe -124	-	expression tag
Glu -123	-	expression tag
Lys -122	-	expression tag
Ser -121	-	expression tag
Ala -120	-	expression tag
Gly -119	-	expression tag
Pro -118	-	expression tag
Pro -117	-	expression tag
Gly -116	-	expression tag
Pro -115	-	expression tag
Pro -114	-	expression tag
Gly -113	-	expression tag
Pro -112	-	expression tag
Pro -111	-	expression tag
Gln 148	Asn 1267	conflict

D: 1 - 424 (UniProt: Q15113)

PDB	External Database	Details
His 425	-	expression tag
His 426	-	expression tag
His 427	-	expression tag
His 428	-	expression tag
His 429	-	expression tag
His 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag
Gly 433	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	170115.9
Non-Polymers*	Number of molecules	5
	Total formula weight	200.4
All*	Total formula weight	170316.3

*Water molecules are not included.