9S9Z
Zn-soaked variant H565A of [FeFe]-hydrogenase CpI from Clostridium pasteurianum
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Iron hydrogenase 1 | polymer | 584 | 65044.3 | 2 | UniProt (P29166) | Clostridium pasteurianum | CpI,Fe-only hydrogenase,[Fe] hydrogenase |
| 2 | C, V (A, B) | dicarbonyl[bis(cyanide-kappaC)]-mu-(iminodimethanethiolatato-1kappaS:2kappaS)-mu-(oxomethylidene)diiron(2+) | non-polymer | 355.0 | 2 | Chemie (402) | |||
| 3 | D, E, F, G, W... (A, B) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 8 | Chemie (SF4) | |||
| 4 | AA, H (B, A) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 2 | Chemie (FES) | |||
| 5 | BA, I, U (B, A) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) PubChem (888) | |||
| 6 | CA, J (B, A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) PubChem (753) | |||
| 7 | DA, EA, FA, GA, HA... (B, A) | ZINC ION | non-polymer | 65.4 | 10 | Chemie (ZN) PubChem (32051) | |||
| 8 | IA, JA, KA, LA, MA... (B, A) | CHLORIDE ION | non-polymer | 35.5 | 10 | Chemie (CL) PubChem (312) | |||
| 9 | NA, OA (A, B) | water | water | 18.0 | 787 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 574 (UniProt: P29166)
| PDB | External Database | Details |
|---|---|---|
| Ala 565 | His 565 | engineered mutation |
| Ser 575 | - | expression tag |
| Ala 576 | - | expression tag |
| Trp 577 | - | expression tag |
| Ser 578 | - | expression tag |
| His 579 | - | expression tag |
| Pro 580 | - | expression tag |
| Gln 581 | - | expression tag |
| Phe 582 | - | expression tag |
| Glu 583 | - | expression tag |
| Lys 584 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 130088.7 | |
| Non-Polymers* | Number of molecules | 37 |
| Total formula weight | 5140.4 | |
| All* | Total formula weight | 135229.1 |
