9S6U

Ternary cryo-EM structure of human ALG9 with Dol25-PP-GlcNAc2Man8, Dol25-P-Man and Fab

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Alpha-1,2-mannosyltransferase ALG9	polymer	617	70347.5	1	UniProt (Q9H6U8)	Homo sapiens (human)	Asparagine-linked glycosylation protein 9 homolog,Disrupted in bipolar disorder protein 1,Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase,Dol-P-Man:Man(8)GlcNAc(2)-PP-Dol alpha-1
2	B (H)	Hs9-8 Fab heavy chain	polymer	240	25638.4	1		synthetic construct
3	C (L)	Hs9-8 Fab light chain	polymer	215	23258.8	1		synthetic construct
4	D (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
5	E (A)	[(3S,6Z,10E,14E)-3,7,11,15,19-pentamethylicosa-6,10,14,18-tetraenyl] dihydrogen phosphate	non-polymer		440.6	1	Chemie (A1JMT)
6	F (A)	[(2~{R},3~{R},4~{R},5~{S},6~{R})-3-acetamido-5-[(2~{S},3~{R},4~{R},5~{S},6~{R})-3-acetamido-6-(hydroxymethyl)-5-[(2~{S},3~{S},4~{S},5~{R},6~{R})-4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3-[(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-4,5-bis(oxidanyl)oxan-2-yl]oxy-6-[[(2~{S},3~{S},4~{S},5~{R},6~{R})-4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3-[(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-4,5-bis(oxidanyl)oxan-2-yl]oxy-6-[[(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxymethyl]-3,5-bis(oxidanyl)oxan-2-yl]oxymethyl]-3,5-bis(oxidanyl)oxan-2-yl]oxy-4-oxidanyl-oxan-2-yl]oxy-6-(hydroxymethyl)-4-oxidanyl-oxan-2-yl] [oxidanyl-[(3~{S},6~{Z},10~{E},14~{E})-3,7,11,15,19-pentamethylicosa-6,10,14,18-tetraenoxy]phosphoryl] hydrogen phosphate	non-polymer		2061.9	1	Chemie (A1JMB)
7	G, H, I (A)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	3	Chemie (Y01)
8	J (A)	water	water		18.0	20	Chemie (HOH)

Sequence modifications

A: 1 - 611 (UniProt: Q9H6U8)

PDB	External Database	Details
Gly -5	-	expression tag
Pro -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Thr 0	-	expression tag
Ala 82	Asp 82	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	119244.7
Non-Polymers*	Number of molecules	6
	Total formula weight	4183.9
All*	Total formula weight	123428.6

*Water molecules are not included.