9S6R
Ternary cryo-EM structure of yeast ALG3 with Dol25-PP-GlcNAc2Man5, Dol25-P-Man, and Fab
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (H) | Sc-16 Fab heavy chain | polymer | 240 | 25714.6 | 1 | synthetic construct | ||
| 2 | B (L) | Sc-16 Fab light chain | polymer | 215 | 23258.8 | 1 | synthetic construct | ||
| 3 | C (A) | Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase | polymer | 467 | 53925.9 | 1 | UniProt (P38179) | Saccharomyces cerevisiae (brewer's yeast) | Asparagine-linked glycosylation protein 3,Dol-P-Man-dependent alpha(1-3)-mannosyltransferase,Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase,Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase,HM-1 killer toxin resistance protein |
| 4 | D (A) | [(2~{R},3~{R},4~{R},5~{S},6~{R})-3-acetamido-5-[(2~{S},3~{R},4~{R},5~{S},6~{R})-3-acetamido-6-(hydroxymethyl)-5-[(2~{S},3~{S},4~{S},5~{R},6~{R})-4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3-[(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-4,5-bis(oxidanyl)oxan-2-yl]oxy-6-[[(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxymethyl]-3,5-bis(oxidanyl)oxan-2-yl]oxy-4-oxidanyl-oxan-2-yl]oxy-6-(hydroxymethyl)-4-oxidanyl-oxan-2-yl] [oxidanyl-[(3~{S},6~{Z},10~{E},14~{E})-3,7,11,15,19-pentamethylicosa-6,10,14,18-tetraenoxy]phosphoryl] hydrogen phosphate | non-polymer | 1575.5 | 1 | Chemie (A1JMA) | |||
| 5 | E (A) | CHOLESTEROL HEMISUCCINATE | non-polymer | 486.7 | 1 | Chemie (Y01) | |||
| 6 | F (A) | Dolichol monophosphate beta-D-Mannose | non-polymer | 602.7 | 1 | Chemie (IZY) | |||
| 7 | G (A) | water | water | 18.0 | 10 | Chemie (HOH) |
Sequence modifications
A: 1 - 458 (UniProt: P38179)
| PDB | External Database | Details |
|---|---|---|
| Asn 71 | Asp 71 | engineered mutation |
| Val 459 | - | expression tag |
| Asp 460 | - | expression tag |
| Ala 461 | - | expression tag |
| Leu 462 | - | expression tag |
| Glu 463 | - | expression tag |
| Val 464 | - | expression tag |
| Leu 465 | - | expression tag |
| Phe 466 | - | expression tag |
| Gln 467 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 102899.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 2665.0 | |
| All* | Total formula weight | 105564.3 |
