9S5U
Cryo-EM structure of yeast EMC:Spf1 insertase:dislocase complex in E1-ATP conformation in digitonin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | ER membrane protein complex subunit 1 | polymer | 760 | 87272.9 | 1 | UniProt (P25574) | Saccharomyces cerevisiae (brewer's yeast) | |
| 2 | B (B) | ER membrane protein complex subunit 2 | polymer | 292 | 33893.2 | 1 | UniProt (P47133) | Saccharomyces cerevisiae (brewer's yeast) | |
| 3 | C (C) | ER membrane protein complex subunit 3 | polymer | 253 | 28372.8 | 1 | UniProt (P36039) | Saccharomyces cerevisiae (brewer's yeast) | Altered inheritance rate of mitochondria protein 27 |
| 4 | D (D) | ER membrane protein complex subunit 4 | polymer | 190 | 21478.7 | 1 | UniProt (P53073) | Saccharomyces cerevisiae (brewer's yeast) | |
| 5 | E (E) | ER membrane protein complex subunit 5 | polymer | 169 | 18799.4 | 1 | UniProt (P40540) | Saccharomyces cerevisiae (brewer's yeast) | Killer toxin-resistance protein 27 |
| 6 | F (F) | ER membrane protein complex subunit 6 | polymer | 108 | 12411.4 | 1 | UniProt (Q12431) | Saccharomyces cerevisiae (brewer's yeast) | |
| 7 | G (G) | Protein SOP4 | polymer | 234 | 26627.6 | 1 | UniProt (P39543) | Saccharomyces cerevisiae (brewer's yeast) | Suppressor of PMA1-7 protein 4 |
| 8 | H (H) | Endoplasmic reticulum membrane protein complex subunit 10 | polymer | 205 | 22792.8 | 1 | UniProt (Q12025) | Saccharomyces cerevisiae (brewer's yeast) | |
| 9 | I (I) | Endoplasmic reticulum transmembrane helix translocase | polymer | 1227 | 136700.2 | 1 | UniProt (P39986) | Saccharomyces cerevisiae (brewer's yeast) | Complexed with DOR1 protein 1,Endoplasmic reticulum P5A-ATPase,Sensitivity to the P.farinosa killer toxin protein 1 |
| 10 | J (Z) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 11 | K, L, M, N, O (A, G) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 5 | Chemie (NAG) PubChem (24139) | |||
| 12 | P (I) | Digitonin | non-polymer | 1229.3 | 1 | Chemie (AJP) PubChem (6474107) |
Sequence modifications
E: 1 - 141 (UniProt: P40540)
I: 1 - 1215 (UniProt: P39986)
| PDB | External Database | Details |
|---|---|---|
| Trp 142 | - | expression tag |
| Ser 143 | - | expression tag |
| His 144 | - | expression tag |
| Pro 145 | - | expression tag |
| Gln 146 | - | expression tag |
| Phe 147 | - | expression tag |
| Glu 148 | - | expression tag |
| Lys 149 | - | expression tag |
| Gly 150 | - | expression tag |
| Gly 151 | - | expression tag |
| Gly 152 | - | expression tag |
| Ser 153 | - | expression tag |
| Gly 154 | - | expression tag |
| Gly 155 | - | expression tag |
| Gly 156 | - | expression tag |
| Ser 157 | - | expression tag |
| Gly 158 | - | expression tag |
| Gly 159 | - | expression tag |
| Ser 160 | - | expression tag |
| Ala 161 | - | expression tag |
| Trp 162 | - | expression tag |
| Ser 163 | - | expression tag |
| His 164 | - | expression tag |
| Pro 165 | - | expression tag |
| Gln 166 | - | expression tag |
| Phe 167 | - | expression tag |
| Glu 168 | - | expression tag |
| Lys 169 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 1216 | - | expression tag |
| Ser 1217 | - | expression tag |
| Ser 1218 | - | expression tag |
| Gly 1219 | - | expression tag |
| Asp 1220 | - | expression tag |
| Tyr 1221 | - | expression tag |
| Lys 1222 | - | expression tag |
| Asp 1223 | - | expression tag |
| Asp 1224 | - | expression tag |
| Asp 1225 | - | expression tag |
| Asp 1226 | - | expression tag |
| Lys 1227 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 388349.1 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 424.4 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 2335.4 | |
| All* | Total formula weight | 391108.9 |
