9S4Q

Cryo-EM structure of the Saccharomyces cerevisiae KMN junction complex lacking the Mis12c(Mtw1c) head 2 domain

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (Nn)	Kinetochore-associated protein NNF1	polymer	201	23668.5	1	UniProt (P47149)	Saccharomyces cerevisiae S288C
2	B (Mt)	Kinetochore-associated protein MTW1	polymer	289	33291.2	1	UniProt (P39731)	Saccharomyces cerevisiae S288C	Mis12-like protein
3	C (Zw)	Outer kinetochore KNL1 complex subunit KRE28	polymer	420	48468.3	1	UniProt (Q04431)	Saccharomyces cerevisiae S288C	Spindle pole body component KRE28
4	D (Kl)	Outer kinetochore KNL1 complex subunit SPC105	polymer	474	55581.0	1	UniProt (P53148)	Saccharomyces cerevisiae S288C	105 kDa spindle pole component protein,Spindle pole body component SPC105
5	E (Ds)	Kinetochore-associated protein DSN1	polymer	576	65777.0	1	UniProt (P40568)	Saccharomyces cerevisiae S288C
6	F (24)	Kinetochore protein SPC24	polymer	213	24639.8	1	UniProt (Q04477)	Saccharomyces cerevisiae S288C
7	G (25)	Kinetochore protein SPC25	polymer	221	25280.3	1	UniProt (P40014)	Saccharomyces cerevisiae S288C
8	H (Ns)	Kinetochore-associated protein NSL1	polymer	216	25448.4	1	UniProt (Q12143)	Saccharomyces cerevisiae S288C

Sequence modifications

Zw: 1 - 385 (UniProt: Q04431)

PDB	External Database	Details
Glu 386	-	expression tag
Asn 387	-	expression tag
Leu 388	-	expression tag
Tyr 389	-	expression tag
Phe 390	-	expression tag
Gln 391	-	expression tag
Ser 392	-	expression tag
Trp 393	-	expression tag
Ser 394	-	expression tag
His 395	-	expression tag
Pro 396	-	expression tag
Gln 397	-	expression tag
Phe 398	-	expression tag
Glu 399	-	expression tag
Lys 400	-	expression tag
Gly 401	-	expression tag
Gly 402	-	expression tag
Gly 403	-	expression tag
Ser 404	-	expression tag
Gly 405	-	expression tag
Gly 406	-	expression tag
Gly 407	-	expression tag
Ser 408	-	expression tag
Gly 409	-	expression tag
Gly 410	-	expression tag
Gly 411	-	expression tag
Ser 412	-	expression tag
Trp 413	-	expression tag
Ser 414	-	expression tag
His 415	-	expression tag
Pro 416	-	expression tag
Gln 417	-	expression tag
Phe 418	-	expression tag
Glu 419	-	expression tag
Lys 420	-	expression tag

Kl: 445 - 917 (UniProt: P53148)

PDB	External Database	Details
Met 444	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	302154.6
All*	Total formula weight	302154.6

*Water molecules are not included.