9S0U
State 3 MAP 3 RNA Pol II activated elongation complex with SETD2 bound to distal upstream H3
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (D) | RNA polymerase II subunit D | polymer | 142 | 16331.3 | 1 | UniProt (A0A287ADR4) Pfam (PF03874) | Sus scrofa (pig) | |
| 2 | B (E) | DNA-directed RNA polymerase II subunit E | polymer | 210 | 24644.3 | 1 | UniProt (I3LSI7) Pfam (PF03871) Pfam (PF01191) | Sus scrofa (pig) | RPB5 homolog |
| 3 | C (F) | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 127 | 14477.0 | 1 | UniProt (A0A8D1KNW4) | Sus scrofa (pig) | |
| 4 | D (G) | DNA-directed RNA polymerase II subunit RPB7 | polymer | 172 | 19314.3 | 1 | UniProt (A0A4X1VKG7) | Sus scrofa (pig) | |
| 5 | E (H) | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 150 | 17162.3 | 1 | UniProt (I3LCB2) Pfam (PF03870) | Sus scrofa (pig) | |
| 6 | F (I) | DNA-directed RNA polymerase II subunit RPB9 | polymer | 125 | 14541.2 | 1 | UniProt (P60899) Pfam (PF02150) Pfam (PF01096) | Sus scrofa (pig) | RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5 |
| 7 | G (J) | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 67 | 7655.1 | 1 | UniProt (A0A4X1VYD0) | Sus scrofa (pig) | |
| 8 | H (K) | DNA-directed RNA polymerase II subunit RPB11-a | polymer | 117 | 13310.3 | 1 | UniProt (F1RKE4) Pfam (PF13656) | Sus scrofa (pig) | |
| 9 | I (L) | RNA polymerase II subunit K | polymer | 58 | 7018.2 | 1 | UniProt (A0A8D0JYF1) | Sus scrofa (pig) | |
| 10 | J (P) | RNA (46-MER) | polymer | 46 | 14843.9 | 1 | synthetic construct | ||
| 11 | K (Q) | RNA polymerase-associated protein CTR9 homolog | polymer | 1179 | 134510.2 | 1 | UniProt (Q6PD62) Pfam (PF13374) Pfam (PF13181) | Homo sapiens (human) | SH2 domain-binding protein 1 |
| 12 | L (T) | Template DNA | polymer | 197 | 60522.5 | 1 | synthetic construct | ||
| 13 | M (W) | WD repeat-containing protein 61 | polymer | 305 | 33617.5 | 1 | UniProt (Q9GZS3) Pfam (PF00400) | Homo sapiens (human) | Meiotic recombination REC14 protein homolog,SKI8 homolog,Ski8 |
| 14 | N (X) | Parafibromin | polymer | 531 | 60673.5 | 1 | UniProt (Q6P1J9) Pfam (PF16050) Pfam (PF05179) | Homo sapiens (human) | Cell division cycle protein 73 homolog,Hyperparathyroidism 2 protein |
| 15 | O (Y) | Transcription elongation factor SPT4 | polymer | 121 | 13508.5 | 1 | UniProt (Q4R941) Pfam (PF06093) | Homo sapiens (human) | DRB sensitivity-inducing factor small subunit,DSIF small subunit |
| 16 | P, T (a, e) | Histone H3.2 | polymer | 136 | 15391.1 | 2 | UniProt (Q71DI3) Pfam (PF00125) | Homo sapiens (human) | H3-clustered histone 13,H3-clustered histone 14,H3-clustered histone 15,Histone H3/m,Histone H3/o |
| 17 | Q, U (b, f) | Histone H4 | polymer | 103 | 11394.4 | 2 | UniProt (P62805) Pfam (PF15511) | Homo sapiens (human) | |
| 18 | R, V (c, g) | Histone H2A type 1-B/E | polymer | 135 | 14721.1 | 2 | UniProt (P04908) Pfam (PF00125) Pfam (PF16211) | Homo sapiens (human) | Histone H2A.2,Histone H2A/a,Histone H2A/m |
| 19 | S, W (d, h) | Histone H2B type 1-K | polymer | 126 | 13921.2 | 2 | UniProt (O60814) Pfam (PF00125) | Homo sapiens (human) | H2B K,HIRA-interacting protein 1 |
| 20 | X (k) | FACT complex subunit SPT16 | polymer | 1049 | 120291.0 | 1 | UniProt (Q9Y5B9) Pfam (PF14826) Pfam (PF00557) Pfam (PF08644) Pfam (PF24824) Pfam (PF08512) Pfam (PF21091) | Homo sapiens (human) | Chromatin-specific transcription elongation factor 140 kDa subunit,FACT 140 kDa subunit,FACTp140,Facilitates chromatin transcription complex subunit SPT16,hSPT16 |
| 21 | Y (A) | DNA-directed RNA polymerase subunit | polymer | 1970 | 217610.0 | 1 | UniProt (A0A8D1DPV6) | Sus scrofa (pig) | |
| 22 | Z (B) | DNA-directed RNA polymerase subunit beta | polymer | 1174 | 134041.4 | 1 | UniProt (I3LGP4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) | Sus scrofa (pig) | |
| 23 | AA (C) | DNA-directed RNA polymerase II subunit RPB3 | polymer | 275 | 31439.1 | 1 | UniProt (I3LCH3) Pfam (PF01193) Pfam (PF01000) | Sus scrofa (pig) | |
| 24 | BA (M) | Transcription elongation factor SPT6 | polymer | 1729 | 199603.0 | 1 | UniProt (Q7KZ85) Pfam (PF14632) Pfam (PF14641) Pfam (PF22706) Pfam (PF14639) Pfam (PF14635) Pfam (PF17674) Pfam (PF00575) Pfam (PF14633) | Homo sapiens (human) | hSPT6,Histone chaperone suppressor of Ty6,Tat-cotransactivator 2 protein,Tat-CT2 protein |
| 25 | CA (N) | Non-template DNA | polymer | 197 | 61155.9 | 1 | synthetic construct | ||
| 26 | DA (O) | Histone-lysine N-methyltransferase SETD2 | polymer | 1133 | 127887.3 | 1 | UniProt (Q9BYW2) Pfam (PF17907) Pfam (PF00856) Pfam (PF00397) Pfam (PF08236) | Homo sapiens (human) | HIF-1,Huntingtin yeast partner B,Huntingtin-interacting protein 1,HIP-1,Huntingtin-interacting protein B,Lysine N-methyltransferase 3A,Protein-lysine N-methyltransferase SETD2,SET domain-containing protein 2,hSET2,p231HBP |
| 27 | EA (R) | RNA polymerase-associated protein RTF1 homolog | polymer | 713 | 80733.0 | 1 | UniProt (Q92541) Pfam (PF03126) | Homo sapiens (human) | |
| 28 | FA (S) | Transcription elongation factor A protein 1 | polymer | 304 | 34295.0 | 1 | UniProt (P23193) Pfam (PF08711) Pfam (PF07500) Pfam (PF01096) | Homo sapiens (human) | Transcription elongation factor S-II protein 1,Transcription elongation factor TFIIS.o |
| 29 | GA (U) | RNA polymerase-associated protein LEO1 | polymer | 666 | 75514.2 | 1 | UniProt (Q8WVC0) Pfam (PF04004) | Homo sapiens (human) | Replicative senescence down-regulated leo1-like protein |
| 30 | HA (V) | RNA polymerase II-associated factor 1 homolog | polymer | 531 | 60052.7 | 1 | UniProt (Q8N7H5) Pfam (PF03985) | Homo sapiens (human) | hPAF1,Pancreatic differentiation protein 2 |
| 31 | IA (Z) | Transcription elongation factor SPT5 | polymer | 1087 | 121225.5 | 1 | UniProt (O00267) Pfam (PF11942) Pfam (PF03439) Pfam (PF00467) Pfam (PF23037) Pfam (PF23288) Pfam (PF23287) | Homo sapiens (human) | hSPT5,DRB sensitivity-inducing factor 160 kDa subunit,DSIF p160,DRB sensitivity-inducing factor large subunit,DSIF large subunit,Tat-cotransactivator 1 protein,Tat-CT1 protein |
| 32 | JA, KA, LA, MA, NA... (I, J, L, Y, A...) | ZINC ION | non-polymer | 65.4 | 9 | Chemie (ZN) | |||
| 33 | QA (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
Q: 1 - 1173 (UniProt: Q6PD62)
Y: 1 - 117 (UniProt: Q4R941)
a, e: 0 - 135 (UniProt: Q71DI3)
c, g: 0 - 129 (UniProt: P04908)
k: 1 - 1047 (UniProt: Q9Y5B9)
M: 1 - 1726 (UniProt: Q7KZ85)
O: 1572 - 2564 (UniProt: Q9BYW2)
R: 1 - 710 (UniProt: Q92541)
S: 1 - 301 (UniProt: P23193)
| PDB | External Database | Details |
|---|---|---|
| Glu 1174 | - | expression tag |
| Asn 1175 | - | expression tag |
| Leu 1176 | - | expression tag |
| Tyr 1177 | - | expression tag |
| Phe 1178 | - | expression tag |
| Gln 1179 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 36 | Lys 37 | engineered mutation |
| Ala 110 | Cys 111 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser -5 | - | expression tag |
| Asn -4 | - | expression tag |
| Ala -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Trp -1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser -1 | - | expression tag |
| Asn 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 1569 | - | expression tag |
| Asn 1570 | - | expression tag |
| Ala 1571 | - | expression tag |
| Leu 1843 | Pro 1962 | variant |
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 35 |
| Total formula weight | 1826833.7 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 613.0 | |
| All* | Total formula weight | 1827446.7 |
