9S0T

Superfolder green fluorescent protein (sfGFP) exhibiting p-(phenylazo)-L-phenylalanine (Pap) at position 39 in complex with alpha-cyclodextrin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Green fluorescent protein	polymer	245	28006.5	2	UniProt (P42212)	Aequorea victoria
2	C, D (C, D)	Cyclohexakis-(1-4)-(alpha-D-glucopyranose)	branched		990.9	2	In PDB GlyTouCan (G22307HL)
3	E (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	F, G (A, B)	water	water		18.0	219	Chemie (HOH)

Sequence modifications

A, B: 2 - 238 (UniProt: P42212)

PDB	External Database	Details
Arg 30	Ser 30	engineered mutation
Ozw 39	Tyr 39	engineered mutation
Leu 64	Phe 64	engineered mutation
Cro 66	Ser 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Arg 80	Gln 80	engineered mutation
Ser 99	Phe 99	engineered mutation
Thr 105	Asn 105	engineered mutation
Phe 145	Tyr 145	engineered mutation
Thr 153	Met 153	engineered mutation
Ala 163	Val 163	engineered mutation
Val 171	Ile 171	engineered mutation
Val 206	Ala 206	engineered mutation
Gln 238	Lys 238	engineered mutation
Ser 239	-	expression tag
Ala 240	-	expression tag
Trp 241	-	expression tag
Ser 242	-	expression tag
His 243	-	expression tag
Pro 244	-	expression tag
Gln 245	-	expression tag
Phe 246	-	expression tag
Glu 247	-	expression tag
Lys 248	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	56012.9
Branched	Number of molecules	2
	Total formula weight	1981.7
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	58086.7

*Water molecules are not included.