9RY2
Crystal structure of a sialic acid binding protein, R113K:G213Q:Q216N mutant, from Streptococcus pneumoniae bound to Neu5Ac
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Sugar ABC transporter, sugar-binding protein | polymer | 439 | 48341.0 | 2 | UniProt (A0A0H2URD1) Pfam (PF01547) | Streptococcus pneumoniae TIGR4 | |
| 2 | C, H (A, B) | N-acetyl-alpha-neuraminic acid | non-polymer | 309.3 | 2 | Chemie (SIA) PubChem (444885) | |||
| 3 | D, E (A) | N-acetyl-beta-neuraminic acid | non-polymer | 309.3 | 2 | Chemie (SLB) PubChem (445063) | |||
| 4 | F (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 5 | G (A) | CITRIC ACID | non-polymer | 192.1 | 1 | Chemie (CIT) PubChem (311) | |||
| 6 | I, J (A, B) | water | water | 18.0 | 549 | Chemie (HOH) |
Sequence modifications
A, B: 23 - 442 (UniProt: A0A0H2URD1)
| PDB | External Database | Details |
|---|---|---|
| Met 4 | - | initiating methionine |
| Ala 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| His 8 | - | expression tag |
| His 9 | - | expression tag |
| His 10 | - | expression tag |
| His 11 | - | expression tag |
| Ser 12 | - | expression tag |
| Ser 13 | - | expression tag |
| Gly 14 | - | expression tag |
| Leu 15 | - | expression tag |
| Glu 16 | - | expression tag |
| Val 17 | - | expression tag |
| Leu 18 | - | expression tag |
| Phe 19 | - | expression tag |
| Gln 20 | - | expression tag |
| Gly 21 | - | expression tag |
| Pro 22 | - | expression tag |
| Lys 113 | Arg 113 | engineered mutation |
| Gln 213 | Gly 213 | engineered mutation |
| Asn 216 | Gln 216 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 96682.0 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1525.3 | |
| All* | Total formula weight | 98207.3 |
