9RX1

Cryo-EM structure of a single-chain beta1-adrenoceptor - AmpC beta-lactamase fusion protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta-1 adrenergic receptor,Beta-lactamase	polymer	654	72983.8	1	UniProt (P07700) UniProt (P00811) Pfam (PF00001) Pfam (PF00144)	Meleagris gallopavo (turkey)	Beta-1 adrenoreceptor,Beta-1 adrenoceptor,Beta-T,Cephalosporinase,CSase
2	B (A)	Cyanopindolol	non-polymer		287.4	1	Chemie (P32) PubChem (46937143)

Sequence modifications

A: 33 - 236 (UniProt: P07700)

PDB	External Database	Details
Met 31	-	initiating methionine
Gly 32	-	expression tag
Ser 68	Arg 68	engineered mutation
Val 90	Met 90	engineered mutation
Leu 116	Cys 116	engineered mutation
Val 129	Ile 129	engineered mutation
Glu 200	Asp 200	engineered mutation
Ala 227	Tyr 227	engineered mutation

A: 237 - 1376 (UniProt: P00811)
A: 284 - 368 (UniProt: P07700)

PDB	External Database	Details
Lys 322	Asp 322	engineered mutation
Ala 327	Phe 327	engineered mutation
Met 338	Phe 338	engineered mutation
Leu 343	Tyr 343	engineered mutation
Ala 358	Cys 358	engineered mutation
Gly 369	-	expression tag
Ser 370	-	expression tag
Gly 371	-	expression tag
Leu 372	-	expression tag
Glu 373	-	expression tag
Val 374	-	expression tag
Leu 375	-	expression tag
Phe 376	-	expression tag
Gln 377	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	72983.8
Non-Polymers*	Number of molecules	1
	Total formula weight	287.4
All*	Total formula weight	73271.1

*Water molecules are not included.