9RUJ

Streptococcus pneumoniae StkP catalytic domain T167E/T169E double mutant in complex with AMP-PNP and Mn2+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Serine/threonine-protein kinase StkP	polymer	309	34686.5	2	UniProt (Q8DNS0)	Streptococcus pneumoniae R6	Ser/Thr-protein kinase StkP,Eukaryotic-type Ser/Thr protein kinase,ESTPK
2	C (B)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)
3	D (B)	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN) PubChem (27854)
4	E, F (A, B)	water	water		18.0	266	Chemie (HOH)

Sequence modifications

A, B: 1 - 287 (UniProt: Q8DNS0)

PDB	External Database	Details
Met -21	-	initiating methionine
Gly -20	-	expression tag
Gly -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Gly -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Arg -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Glu 167	Thr 167	engineered mutation
Glu 169	Thr 169	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	69372.9
Non-Polymers*	Number of molecules	2
	Total formula weight	561.1
All*	Total formula weight	69934.0

*Water molecules are not included.