9RQJ

Cryo-EM structure of the SbmA V102G mutant variant in an expanded outward-open conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Peptide antibiotic transporter SbmA	polymer	425	48790.5	2	UniProt (P0AFY6)	Escherichia coli
2	C, E (A, B)	(1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE	non-polymer		751.0	2	Chemie (PGT)
3	D, F (A, B)	DODECYL-BETA-D-MALTOSIDE	non-polymer		510.6	2	Chemie (LMT) PubChem (114880)

Sequence modifications

A, B: 2 - 406 (UniProt: P0AFY6)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 102	Val 102	engineered mutation
Ala 407	-	expression tag
Leu 408	-	expression tag
Glu 409	-	expression tag
Val 410	-	expression tag
Leu 411	-	expression tag
Phe 412	-	expression tag
Gln 413	-	expression tag
Gly 414	-	expression tag
Pro 415	-	expression tag
His 416	-	expression tag
His 417	-	expression tag
His 418	-	expression tag
His 419	-	expression tag
His 420	-	expression tag
His 421	-	expression tag
His 422	-	expression tag
His 423	-	expression tag
His 424	-	expression tag
His 425	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	97581.0
Non-Polymers*	Number of molecules	4
	Total formula weight	2523.3
All*	Total formula weight	100104.3

*Water molecules are not included.