9RNJ
Cryo-EM structure of the human potassium chloride cotransporter T906A/T1007A phospho-knockout mutants KCC2b bound ATP in LMNG (outward-facing state, dimer)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (B, A) | Isoform 2 of Solute carrier family 12 member 5 | polymer | 1166 | 129059.8 | 2 | UniProt (Q9H2X9) | Homo sapiens (human) | Electroneutral potassium-chloride cotransporter 2,K-Cl cotransporter 2,hKCC2,Neuronal K-Cl cotransporter |
| 2 | C, H (B, A) | N-cyclopropyl-N-(4-methyl-1,3-thiazol-2-yl)-2-[(6-phenylpyridazin-3-yl)sulfanyl]acetamide | non-polymer | 382.5 | 2 | Chemie (JUX) PubChem (70690453) | |||
| 3 | D, I (B, A) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
| 4 | E, J (B, A) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) PubChem (312) | |||
| 5 | F, G, K, L (B, A) | SODIUM ION | non-polymer | 23.0 | 4 | Chemie (NA) PubChem (923) | |||
| 6 | M, N (B, A) | water | water | 18.0 | 2 | Chemie (HOH) |
Sequence modifications
B, A: 2 - 1116 (UniProt: Q9H2X9)
| PDB | External Database | Details |
|---|---|---|
| Met -48 | - | initiating methionine |
| Gly -47 | - | expression tag |
| Ser -46 | - | expression tag |
| Ala -45 | - | expression tag |
| Trp -44 | - | expression tag |
| Ser -43 | - | expression tag |
| His -42 | - | expression tag |
| Pro -41 | - | expression tag |
| Gln -40 | - | expression tag |
| Phe -39 | - | expression tag |
| Glu -38 | - | expression tag |
| Lys -37 | - | expression tag |
| Gly -36 | - | expression tag |
| Gly -35 | - | expression tag |
| Gly -34 | - | expression tag |
| Ser -33 | - | expression tag |
| Gly -32 | - | expression tag |
| Gly -31 | - | expression tag |
| Gly -30 | - | expression tag |
| Ser -29 | - | expression tag |
| Gly -28 | - | expression tag |
| Gly -27 | - | expression tag |
| Ser -26 | - | expression tag |
| Ala -25 | - | expression tag |
| Trp -24 | - | expression tag |
| Ser -23 | - | expression tag |
| His -22 | - | expression tag |
| Pro -21 | - | expression tag |
| Gln -20 | - | expression tag |
| Phe -19 | - | expression tag |
| Glu -18 | - | expression tag |
| Lys -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Val -4 | - | expression tag |
| Leu -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Pro 1 | - | expression tag |
| Ala 69 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 258119.5 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1942.2 | |
| All* | Total formula weight | 260061.8 |
