9RIA

Cryo-EM structure of tomato NRC3-AVRcap1b complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	SlNRC3	polymer	919	104764.0	3	UniProt (A0A3Q7GDL1) Pfam (PF18052) Pfam (PF00931) Pfam (PF23559) Pfam (PF23598)	Solanum lycopersicum (tomato)
2	D (G)	RxLR effector protein PITG_16705	polymer	684	78138.4	1	UniProt (D0NVF3) Pfam (PF22748)	Phytophthora infestans (potato late blight agent)
3	E, F, G (A, B, C)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	3	Chemie (ATP)

Sequence modifications

A, B, C: 1 - 891 (UniProt: A0A3Q7GDL1)

PDB	External Database	Details
Glu 9	Leu 9	engineered mutation
Glu 13	Leu 13	engineered mutation
Glu 17	Leu 17	engineered mutation
Ser 892	-	expression tag
Asp 893	-	expression tag
Tyr 894	-	expression tag
Lys 895	-	expression tag
Asp 896	-	expression tag
His 897	-	expression tag
Asp 898	-	expression tag
Gly 899	-	expression tag
Asp 900	-	expression tag
Tyr 901	-	expression tag
Lys 902	-	expression tag
Asp 903	-	expression tag
His 904	-	expression tag
Asp 905	-	expression tag
Leu 906	-	expression tag
Asp 907	-	expression tag
Ala 908	-	expression tag
Ala 909	-	expression tag
Ala 910	-	expression tag
Ala 911	-	expression tag
Asp 912	-	expression tag
Tyr 913	-	expression tag
Lys 914	-	expression tag
Asp 915	-	expression tag
Asp 916	-	expression tag
Asp 917	-	expression tag
Asp 918	-	expression tag
Lys 919	-	expression tag

G: 62 - 678 (UniProt: D0NVF3)

PDB	External Database	Details
Met 61	-	initiating methionine
Glu 92	Pro 92	engineered mutation
Tyr 679	-	expression tag
Ser 680	-	expression tag
Tyr 681	-	expression tag
Pro 682	-	expression tag
Tyr 683	-	expression tag
Asp 684	-	expression tag
Val 685	-	expression tag
Pro 686	-	expression tag
Asp 687	-	expression tag
Tyr 688	-	expression tag
Ala 689	-	expression tag
Gly 690	-	expression tag
Tyr 691	-	expression tag
Pro 692	-	expression tag
Tyr 693	-	expression tag
Asp 694	-	expression tag
Val 695	-	expression tag
Pro 696	-	expression tag
Asp 697	-	expression tag
Tyr 698	-	expression tag
Ala 699	-	expression tag
Gly 700	-	expression tag
Leu 701	-	expression tag
Tyr 702	-	expression tag
Pro 703	-	expression tag
Tyr 704	-	expression tag
Asp 705	-	expression tag
Val 706	-	expression tag
Pro 707	-	expression tag
Asp 708	-	expression tag
Tyr 709	-	expression tag
Ala 710	-	expression tag
Thr 711	-	expression tag
Arg 712	-	expression tag
Ala 713	-	expression tag
Ala 714	-	expression tag
Tyr 715	-	expression tag
Pro 716	-	expression tag
Tyr 717	-	expression tag
Asp 718	-	expression tag
Val 719	-	expression tag
Pro 720	-	expression tag
Asp 721	-	expression tag
Tyr 722	-	expression tag
Ala 723	-	expression tag
Gly 724	-	expression tag
Tyr 725	-	expression tag
Pro 726	-	expression tag
Tyr 727	-	expression tag
Asp 728	-	expression tag
Val 729	-	expression tag
Pro 730	-	expression tag
Asp 731	-	expression tag
Tyr 732	-	expression tag
Ala 733	-	expression tag
Gly 734	-	expression tag
Leu 735	-	expression tag
Tyr 736	-	expression tag
Pro 737	-	expression tag
Tyr 738	-	expression tag
Asp 739	-	expression tag
Val 740	-	expression tag
Pro 741	-	expression tag
Asp 742	-	expression tag
Tyr 743	-	expression tag
Ala 744	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	392430.3
Non-Polymers*	Number of molecules	3
	Total formula weight	1521.5
All*	Total formula weight	393951.8

*Water molecules are not included.