9RHI

pre-Initiation Complex on ARS1 DNA (monomer)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (2)	DNA replication licensing factor MCM2	polymer	868	98911.5	1	UniProt (P29469)	Saccharomyces cerevisiae (brewer's yeast)	Minichromosome maintenance protein 2
2	B (3)	DNA replication licensing factor MCM3	polymer	1006	111720.2	1	UniProt (P24279)	Saccharomyces cerevisiae (brewer's yeast)	Minichromosome maintenance protein 3
3	C (4)	DNA replication licensing factor MCM4	polymer	933	105138.4	1	UniProt (P30665)	Saccharomyces cerevisiae (brewer's yeast)	Cell division control protein 54
4	D (5)	Minichromosome maintenance protein 5	polymer	775	86505.7	1	UniProt (P29496)	Saccharomyces cerevisiae (brewer's yeast)	Cell division control protein 46
5	E (6)	DNA replication licensing factor MCM6	polymer	1017	113110.2	1	UniProt (P53091)	Saccharomyces cerevisiae (brewer's yeast)	Minichromosome maintenance protein 6
6	F, S (7, f)	DNA replication licensing factor MCM7	polymer	845	95049.9	2	UniProt (P38132)	Saccharomyces cerevisiae (brewer's yeast)	Cell division control protein 47,Minichromosome maintenance protein 7
7	G (A)	DNA (31-MER)	polymer	31	9512.2	1		Saccharomyces cerevisiae
8	H (B)	DNA (31-MER)	polymer	31	9547.2	1		Saccharomyces cerevisiae
9	I (C)	DNA replication complex GINS protein PSF3	polymer	217	24437.9	1	UniProt (Q12146)	Saccharomyces cerevisiae (brewer's yeast)	Partner of Sld five 3
10	J (D)	DNA replication complex GINS protein SLD5	polymer	294	33983.6	1	UniProt (Q03406)	Saccharomyces cerevisiae (brewer's yeast)
11	K (E)	Cell division control protein 45	polymer	657	75154.7	1	UniProt (Q08032)	Saccharomyces cerevisiae (brewer's yeast)
12	L (F)	DNA polymerase epsilon subunit B	polymer	689	78425.9	1	UniProt (P24482)	Saccharomyces cerevisiae (brewer's yeast)	DNA polymerase II subunit 2
13	M (H)	DNA replication complex GINS protein PSF1	polymer	208	24230.6	1	UniProt (Q12488)	Saccharomyces cerevisiae (brewer's yeast)	Partner of Sld five 1
14	N (I)	DNA replication complex GINS protein PSF2	polymer	213	25096.8	1	UniProt (P40359)	Saccharomyces cerevisiae (brewer's yeast)	Partner of Sld five 2
15	O, R (Q, T)	DNA replication regulator SLD3	polymer	704	81240.7	2	UniProt (P53135)	Saccharomyces cerevisiae (brewer's yeast)
16	P (R)	Mitochondrial morphogenesis protein SLD7	polymer	257	29598.8	1	UniProt (Q08457)	Saccharomyces cerevisiae (brewer's yeast)	Synthetic lethality with DPB11-24 mutation protein 7
17	Q (S)	DNA replication regulator DPB11	polymer	801	91548.7	1	UniProt (P47027)	Saccharomyces cerevisiae (brewer's yeast)
18	T, U, V, W, Z (2, 4, 5, 6, 7)	ZINC ION	non-polymer		65.4	5	Chemie (ZN) PubChem (32051)
19	X (7)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
20	Y (7)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)

Sequence modifications

3: 1 - 971 (UniProt: P24279)

PDB	External Database	Details
Met -34	-	initiating methionine
Lys -33	-	expression tag
Arg -32	-	expression tag
Arg -31	-	expression tag
Trp -30	-	expression tag
Lys -29	-	expression tag
Lys -28	-	expression tag
Asn -27	-	expression tag
Phe -26	-	expression tag
Ile -25	-	expression tag
Ala -24	-	expression tag
Val -23	-	expression tag
Ser -22	-	expression tag
Ala -21	-	expression tag
Ala -20	-	expression tag
Asn -19	-	expression tag
Arg -18	-	expression tag
Phe -17	-	expression tag
Lys -16	-	expression tag
Lys -15	-	expression tag
Ile -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Glu 0	-	expression tag

C: 1 - 194 (UniProt: Q12146)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

E: 1 - 197 (UniProt: Q08032)
E: 211 - 657 (UniProt: Q08032)

PDB	External Database	Details
Asp 198	-	linker
Tyr 199	-	linker
Lys 200	-	linker
Asp 201	-	linker
Asp 202	-	linker
Asp 203	-	linker
Gly 204	-	linker
Asp 205	-	linker
Tyr 206	-	linker
Lys 207	-	linker
Asp 208	-	linker
Asp 209	-	linker
Asp 210	-	linker

Q, T: 1 - 668 (UniProt: P53135)

PDB	External Database	Details
Glu 669	-	expression tag
Asn 670	-	expression tag
Leu 671	-	expression tag
Tyr 672	-	expression tag
Phe 673	-	expression tag
Gln 674	-	expression tag
Gly 675	-	expression tag
Trp 676	-	expression tag
Ser 677	-	expression tag
His 678	-	expression tag
Pro 679	-	expression tag
Gln 680	-	expression tag
Phe 681	-	expression tag
Glu 682	-	expression tag
Lys 683	-	expression tag
Gly 684	-	expression tag
Gly 685	-	expression tag
Gly 686	-	expression tag
Ser 687	-	expression tag
Gly 688	-	expression tag
Gly 689	-	expression tag
Gly 690	-	expression tag
Ser 691	-	expression tag
Gly 692	-	expression tag
Gly 693	-	expression tag
Ser 694	-	expression tag
Ser 695	-	expression tag
Ala 696	-	expression tag
Trp 697	-	expression tag
Ser 698	-	expression tag
His 699	-	expression tag
Pro 700	-	expression tag
Gln 701	-	expression tag
Phe 702	-	expression tag
Glu 703	-	expression tag
Lys 704	-	expression tag

S: 1 - 764 (UniProt: P47027)

PDB	External Database	Details
Gly 765	-	expression tag
Ser 766	-	expression tag
Ser 767	-	expression tag
Leu 768	-	expression tag
Glu 769	-	expression tag
Val 770	-	expression tag
Leu 771	-	expression tag
Phe 772	-	expression tag
Gln 773	-	expression tag
Gly 774	-	expression tag
Pro 775	-	expression tag
Glu 776	-	expression tag
Gly 777	-	expression tag
Ser 778	-	expression tag
Ser 779	-	expression tag
Asp 780	-	expression tag
Tyr 781	-	expression tag
Lys 782	-	expression tag
Asp 783	-	expression tag
His 784	-	expression tag
Asp 785	-	expression tag
Gly 786	-	expression tag
Asp 787	-	expression tag
Tyr 788	-	expression tag
Lys 789	-	expression tag
Asp 790	-	expression tag
His 791	-	expression tag
Asp 792	-	expression tag
Ile 793	-	expression tag
Asp 794	-	expression tag
Tyr 795	-	expression tag
Lys 796	-	expression tag
Asp 797	-	expression tag
Asp 798	-	expression tag
Asp 799	-	expression tag
Asp 800	-	expression tag
Lys 801	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	19
	Total formula weight	1269503.5
Non-Polymers*	Number of molecules	7
	Total formula weight	778.6
All*	Total formula weight	1270282.1

*Water molecules are not included.