9RH1

Cryo-EM structure of the consensus inward-facing apo NhaA dimer at pH 7.5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Na(+)/H(+) antiporter NhaA	polymer	407	43585.9	2	UniProt (P13738)	Escherichia coli	Na(+)/H(+) exchanger,Na(+)/Li(+)/H(+) antiporter,Sodium/proton antiporter NhaA
2	B, E (B, E)	Fv6F9 heavy chain	polymer	127	13934.4	2		Mus musculus
3	C, F (C, F)	Fv6F9 light chain	polymer	119	13193.6	2		Mus musculus
4	G (A)	CARDIOLIPIN	non-polymer		1464.0	1	Chemie (CDL)
5	H (D)	(1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE	non-polymer		751.0	1	Chemie (PGT)

Sequence modifications

A, D: 1 - 388 (UniProt: P13738)

PDB	External Database	Details
Ala 389	-	expression tag
Ala 390	-	expression tag
Ala 391	-	expression tag
Ile 392	-	expression tag
Glu 393	-	expression tag
Gly 394	-	expression tag
Arg 395	-	expression tag
Ile 396	-	expression tag
Glu 397	-	expression tag
Gly 398	-	expression tag
Arg 399	-	expression tag
Leu 400	-	expression tag
Glu 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag
His 407	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	141427.7
Non-Polymers*	Number of molecules	2
	Total formula weight	2215.1
All*	Total formula weight	143642.7

*Water molecules are not included.