9RCK

Laccase (multicopper oxidase) from Pediococcus pentosaceus 4618 mutant M455A-M456A soaked with Copper 2 minutes

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (C, A, B, E)	Multicopper oxidase domain-containing protein	polymer	532	59733.3	4	UniProt (A0AB73HHR8)	Pediococcus pentosaceus 4618
2	E (C)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
3	F, G, H, I, J... (A, B)	COPPER (II) ION	non-polymer		63.5	21	Chemie (CU)
4	AA, BA, CA, DA (C, A, B, E)	water	water		18.0	236	Chemie (HOH)

Sequence modifications

C, A, B, E: 22 - 530 (UniProt: A0AB73HHR8)

PDB	External Database	Details
Met -1	-	initiating methionine
Gly 0	-	expression tag
Ser 1	-	expression tag
Ser 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
Ser 9	-	expression tag
Ser 10	-	expression tag
Gly 11	-	expression tag
Leu 12	-	expression tag
Val 13	-	expression tag
Pro 14	-	expression tag
Arg 15	-	expression tag
Gly 16	-	expression tag
Ser 17	-	expression tag
His 18	-	expression tag
Met 19	-	expression tag
Ala 20	-	expression tag
Ser 21	-	expression tag
Pro 33	Gln 12	variant
Asn 262	Asp 241	variant
Ala 274	Ser 253	variant
Ala 476	Met 455	engineered mutation
Ala 477	Met 456	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	238933.2
Non-Polymers*	Number of molecules	22
	Total formula weight	1430.5
All*	Total formula weight	240363.7

*Water molecules are not included.