9RCJ

Laccase (multicopper oxidase) from Pediococcus pentosaceus 4618 mutant M455A-M456A soaked with Copper 20 seconds

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, F)	Multicopper oxidase domain-containing protein	polymer	532	59733.3	4	UniProt (A0AB73HHR8)	Pediococcus pentosaceus
2	E, F, G, H, I... (A, B)	COPPER (II) ION	non-polymer		63.5	13	Chemie (CU)
3	L, S (A, C)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	T, U, V, W (A, B, C, F)	water	water		18.0	179	Chemie (HOH)

Sequence modifications

A, B, C, F: 1 - 509 (UniProt: A0AB73HHR8)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Pro 12	Gln 12	variant
Asn 241	Asp 241	variant
Ala 253	Ser 253	variant
Ala 455	Met 455	engineered mutation
Ala 456	Met 456	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	238933.2
Non-Polymers*	Number of molecules	15
	Total formula weight	1018.2
All*	Total formula weight	239951.4

*Water molecules are not included.