9RAG

Influenza A/H7N9 polymerase in complex with a 70-mer RNA template, in stalled elongation.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Polymerase acidic protein	polymer	716	82829.6	1	UniProt (M9TI86)	Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
2	B (C)	Polymerase basic protein 2	polymer	788	89038.6	1	UniProt (A0A024E1J5)	Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))	RNA-directed RNA polymerase subunit P3
3	C (G)	RNA Pol II CTD 6 repeats (site 1A/2A)	polymer	42	4816.3	1		Homo sapiens
4	D (M)	RNA product	polymer	11	3560.2	1		Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
5	E (B)	RNA-directed RNA polymerase catalytic subunit	polymer	757	86424.0	1	UniProt (S5ME50)	Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
6	F (T)	RNA template (70-mer)	polymer	70	22480.3	1		Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
7	G, H, I (A, B)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
8	J (B)	PYROPHOSPHATE 2-	non-polymer		176.0	1	Chemie (POP)
9	K, L, M, N, O... (A, C, G, M, B...)	water	water		18.0	667	Chemie (HOH)

Sequence modifications

A: 1 - 716 (UniProt: M9TI86)

PDB	External Database	Details
Lys 349	Glu 349	engineered mutation
Ile 490	Arg 490	engineered mutation

C: 1 - 759 (UniProt: A0A024E1J5)

PDB	External Database	Details
Arg 74	Gly 74	conflict
Gly 760	-	expression tag
Trp 761	-	expression tag
Ser 762	-	expression tag
His 763	-	expression tag
Pro 764	-	expression tag
Gln 765	-	expression tag
Phe 766	-	expression tag
Glu 767	-	expression tag
Lys 768	-	expression tag
Gly 769	-	expression tag
Gly 770	-	expression tag
Gly 771	-	expression tag
Ser 772	-	expression tag
Gly 773	-	expression tag
Gly 774	-	expression tag
Gly 775	-	expression tag
Ser 776	-	expression tag
Gly 777	-	expression tag
Gly 778	-	expression tag
Ser 779	-	expression tag
Ala 780	-	expression tag
Trp 781	-	expression tag
Ser 782	-	expression tag
His 783	-	expression tag
Pro 784	-	expression tag
Gln 785	-	expression tag
Phe 786	-	expression tag
Glu 787	-	expression tag
Lys 788	-	expression tag

B: 1 - 757 (UniProt: S5ME50)

PDB	External Database	Details
Gly 577	Lys 577	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	289149.1
Non-Polymers*	Number of molecules	4
	Total formula weight	248.9
All*	Total formula weight	289398.0

*Water molecules are not included.