9R42

Quinpirole-bound Dopamine D3 Receptor - Gi Protein Complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	352	38810.4	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (R)	Soluble cytochrome b562,D(3) dopamine receptor	polymer	561	62633.1	1	UniProt (P0ABE7) UniProt (P35462) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,Dopamine D3 receptor
5	E (R)	(4aR,8aR)-5-propyl-1,4,4a,6,7,8,8a,9-octahydropyrazolo[3,4-g]quinoline	non-polymer		219.3	1	Chemie (A1JDS)
6	F (R)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -11	-	initiating methionine
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: -116 - -12 (UniProt: P0ABE7)

PDB	External Database	Details
Met -160	-	initiating methionine
Lys -159	-	expression tag
Thr -158	-	expression tag
Ile -157	-	expression tag
Ile -156	-	expression tag
Ala -155	-	expression tag
Leu -154	-	expression tag
Ser -153	-	expression tag
Tyr -152	-	expression tag
Ile -151	-	expression tag
Phe -150	-	expression tag
Cys -149	-	expression tag
Leu -148	-	expression tag
Val -147	-	expression tag
Phe -146	-	expression tag
Ala -145	-	expression tag
Asp -144	-	expression tag
Tyr -143	-	expression tag
Lys -142	-	expression tag
Asp -141	-	expression tag
Asp -140	-	expression tag
Asp -139	-	expression tag
Asp -138	-	expression tag
Ala -137	-	expression tag
Lys -136	-	expression tag
Leu -135	-	expression tag
Gln -134	-	expression tag
Thr -133	-	expression tag
Met -132	-	expression tag
His -131	-	expression tag
His -130	-	expression tag
His -129	-	expression tag
His -128	-	expression tag
His -127	-	expression tag
His -126	-	expression tag
His -125	-	expression tag
His -124	-	expression tag
His -123	-	expression tag
His -122	-	expression tag
His -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
Trp -110	Met 29	engineered mutation
Ile -15	His 124	engineered mutation
Leu -11	-	linker
Ala -10	-	linker
Ser -9	-	linker
Glu -8	-	linker
Asn -7	-	linker
Leu -6	-	linker
Tyr -5	-	linker
Phe -4	-	linker
Gln -3	-	linker
Gly -2	-	linker
Gly -1	-	linker
Thr 0	-	linker

R: 1 - 400 (UniProt: P35462)

PDB	External Database	Details
Ser 9	Gly 9	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	149718.6
Non-Polymers*	Number of molecules	1
	Total formula weight	219.3
All*	Total formula weight	149938.0

*Water molecules are not included.