9QZ1

MINPP1 from Bacteroides thetaiotaomicron E325N mutant complex with myo-inositol(1,2,4,5,6)pentaphosphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	multiple inositol polyphosphate histidine phosphatase 1	polymer	426	49188.4	2	UniProt (Q89YI8) Pfam (PF00328)	Bacteroides thetaiotaomicron VPI-5482	MINPP
2	C, D, E (A, B)	(1R,2R,3R,4S,5R,6R)-6-hydroxycyclohexane-1,2,3,4,5-pentayl pentakis[dihydrogen (phosphate)]	non-polymer		580.1	3	Chemie (ZNZ)
3	F, G (A, B)	water	water		18.0	84	Chemie (HOH)

Sequence modifications

A, B: 21 - 425 (UniProt: Q89YI8)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 1	-	expression tag
Ser 2	-	expression tag
Ser 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
Ser 10	-	expression tag
Ser 11	-	expression tag
Gly 12	-	expression tag
Leu 13	-	expression tag
Val 14	-	expression tag
Pro 15	-	expression tag
Arg 16	-	expression tag
Gly 17	-	expression tag
Ser 18	-	expression tag
His 19	-	expression tag
Met 20	-	expression tag
Asn 325	Glu 325	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	98376.8
Non-Polymers*	Number of molecules	3
	Total formula weight	1740.2
All*	Total formula weight	100117.0

*Water molecules are not included.