9QYS
Crystal structure of leaf branch compost cutinase variant ICCG L50Y Q238K
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Leaf-branch compost cutinase | polymer | 267 | 28917.4 | 1 | UniProt (G9BY57) Pfam (PF12695) | uncultured bacterium | LC-cutinase,LCC,PET-digesting enzyme,Poly(ethylene terephthalate) hydrolase,PET hydrolase,PETase |
| 2 | B, C, D (A) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 3 | E (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 4 | F (A) | water | water | 18.0 | 206 | Chemie (HOH) |
Sequence modifications
A: 2 - 259 (UniProt: G9BY57)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Tyr 50 | Leu 84 | engineered mutation |
| Gly 93 | Tyr 127 | conflict |
| Cys 204 | Asp 238 | conflict |
| Ile 209 | Phe 243 | conflict |
| Lys 238 | Gln 272 | engineered mutation |
| Cys 249 | Ser 283 | conflict |
| Leu 260 | - | expression tag |
| Glu 261 | - | expression tag |
| His 262 | - | expression tag |
| His 263 | - | expression tag |
| His 264 | - | expression tag |
| His 265 | - | expression tag |
| His 266 | - | expression tag |
| His 267 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 28917.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 129.3 | |
| All* | Total formula weight | 29046.7 |
