9QXL

The structure of ADGRL4 in the active-state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Green fluorescent protein,Adhesion G protein-coupled receptor L4	polymer	628	70008.3	1	UniProt (P42212) UniProt (Q9HBW9) Pfam (PF00002)	Homo sapiens (human)	EGF,latrophilin and seven transmembrane domain-containing protein 1,EGF-TM7-latrophilin-related protein,ETL protein
2	B (B)	GNAS complex locus,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	268	30585.2	1	UniProt (Q5JWD1) UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	348	38522.1	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
4	D (E)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I

Sequence modifications

A: 154 - 390 (UniProt: P42212)

PDB	External Database	Details
Met 63	-	initiating methionine
Lys 64	-	expression tag
Arg 65	-	expression tag
Leu 66	-	expression tag
Pro 67	-	expression tag
Leu 68	-	expression tag
Leu 69	-	expression tag
Val 70	-	expression tag
Val 71	-	expression tag
Phe 72	-	expression tag
Ser 73	-	expression tag
Thr 74	-	expression tag
Leu 75	-	expression tag
Leu 76	-	expression tag
Asn 77	-	expression tag
Cys 78	-	expression tag
Ser 79	-	expression tag
Tyr 80	-	expression tag
Thr 81	-	expression tag
Gln 82	-	expression tag
Tyr 83	-	expression tag
Pro 84	-	expression tag
Tyr 85	-	expression tag
Asp 86	-	expression tag
Val 87	-	expression tag
Pro 88	-	expression tag
Asp 89	-	expression tag
Tyr 90	-	expression tag
Ala 91	-	expression tag
Gly 92	-	expression tag
Ser 93	-	expression tag
Gly 94	-	expression tag
Gly 95	-	expression tag
Ser 96	-	expression tag
Ala 97	-	expression tag
Trp 98	-	expression tag
Ser 99	-	expression tag
His 100	-	expression tag
Pro 101	-	expression tag
Gln 102	-	expression tag
Phe 103	-	expression tag
Glu 104	-	expression tag
Lys 105	-	expression tag
Gly 106	-	expression tag
Gly 107	-	expression tag
Gly 108	-	expression tag
Ser 109	-	expression tag
Gly 110	-	expression tag
Gly 111	-	expression tag
Gly 112	-	expression tag
Ser 113	-	expression tag
Gly 114	-	expression tag
Gly 115	-	expression tag
Ser 116	-	expression tag
Ala 117	-	expression tag
Trp 118	-	expression tag
Ser 119	-	expression tag
His 120	-	expression tag
Pro 121	-	expression tag
Gln 122	-	expression tag
Phe 123	-	expression tag
Glu 124	-	expression tag
Lys 125	-	expression tag
Gly 126	-	expression tag
Ser 127	-	expression tag
Gly 128	-	expression tag
His 129	-	expression tag
His 130	-	expression tag
His 131	-	expression tag
His 132	-	expression tag
His 133	-	expression tag
His 134	-	expression tag
His 135	-	expression tag
His 136	-	expression tag
His 137	-	expression tag
His 138	-	expression tag
Gly 139	-	expression tag
Ser 140	-	expression tag
Gly 141	-	expression tag
Leu 142	-	expression tag
Glu 143	-	expression tag
Val 144	-	expression tag
Leu 145	-	expression tag
Phe 146	-	expression tag
Gln 147	-	expression tag
Gly 148	-	expression tag
Pro 149	-	expression tag
Gly 150	-	expression tag
Ser 151	-	expression tag
Gly 152	-	expression tag
Val 153	-	expression tag
Leu 216	Phe 64	conflict
Thr 217	Ser 65	conflict
Lys 358	Ala 206	conflict
Leu 383	His 231	conflict
Gly 391	-	linker
Ser 392	-	linker
Gly 393	-	linker
Glu 394	-	linker
Asn 395	-	linker
Leu 396	-	linker
Tyr 397	-	linker
Phe 398	-	linker
Gln 399	-	linker
Ser 400	-	linker
Gly 401	-	linker
Ser 402	-	linker
Gly 403	-	linker
Gly 404	-	linker
Ser 405	-	linker
Gly 406	-	linker

A: 407 - 690 (UniProt: Q9HBW9)
B: 146 - 205 (UniProt: Q5JWD1)

PDB	External Database	Details
Gly 127	-	expression tag
Gly 128	-	expression tag
Ser 129	-	expression tag
Gly 130	-	expression tag
Ser 131	-	expression tag
Gly 132	-	expression tag
Asp 133	-	expression tag
Tyr 134	-	expression tag
Lys 135	-	expression tag
Asp 136	-	expression tag
Asp 137	-	expression tag
Asp 138	-	expression tag
Asp 139	-	expression tag
Lys 140	-	expression tag
Gly 141	-	expression tag
Gly 142	-	expression tag
Ser 143	-	expression tag
Gly 144	-	expression tag
Ser 145	-	expression tag
Asp 190	Gly 49	conflict
Asn 191	Glu 50	conflict
Gly 206	-	linker
Gly 207	-	linker
Ser 208	-	linker
Gly 209	-	linker
Gly 210	-	linker
Ser 211	-	linker
Gly 212	-	linker
Gly 213	-	linker

B: 214 - 394 (UniProt: P63092)

PDB	External Database	Details
Asp 259	Ala 249	conflict
Asp 262	Ser 252	conflict
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Ala 372	Ile 372	conflict
Ile 375	Val 375	conflict
Lys 380	Arg 380	conflict
Leu 384	Gln 384	conflict
Gln 385	Arg 385	conflict
Asn 387	His 387	conflict
Glu 390	Gln 390	conflict
Asn 392	Glu 392	conflict
Val 394	Leu 394	conflict

C: 1 - 339 (UniProt: P62873)

PDB	External Database	Details
Met -8	-	initiating methionine
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	146976.7
All*	Total formula weight	146976.7

*Water molecules are not included.