9QX1

Cryo-EM structure of the C. elegans UBR4/KCMF1 complex (C-term dimer interface focused refinement)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	UBR-type domain-containing protein	polymer	4284	488244.8	2	UniProt (A0A0S4XR36) Pfam (PF02207) Pfam (PF02916) Pfam (PF13764)	Caenorhabditis elegans
2	B, D (C, D)	KCMF1	polymer	562	62912.8	2	UniProt (P34664) Pfam (PF00569) Pfam (PF05605)	Caenorhabditis elegans
3	E, F (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, B: -6 - 4211 (UniProt: A0A0S4XR36)

PDB	External Database	Details
Gly 4212	-	expression tag
Ser 4213	-	expression tag
Leu 4214	-	expression tag
Glu 4215	-	expression tag
Val 4216	-	expression tag
Leu 4217	-	expression tag
Phe 4218	-	expression tag
Gln 4219	-	expression tag
Gly 4220	-	expression tag
Pro 4221	-	expression tag
Ala 4222	-	expression tag
Glu 4223	-	expression tag
Ala 4224	-	expression tag
Ala 4225	-	expression tag
Ala 4226	-	expression tag
Lys 4227	-	expression tag
Glu 4228	-	expression tag
Ala 4229	-	expression tag
Ala 4230	-	expression tag
Ala 4231	-	expression tag
Lys 4232	-	expression tag
Glu 4233	-	expression tag
Ala 4234	-	expression tag
Ala 4235	-	expression tag
Ala 4236	-	expression tag
Lys 4237	-	expression tag
Glu 4238	-	expression tag
Ala 4239	-	expression tag
Ala 4240	-	expression tag
Ala 4241	-	expression tag
Lys 4242	-	expression tag
Ala 4243	-	expression tag
Leu 4244	-	expression tag
Glu 4245	-	expression tag
Ala 4246	-	expression tag
Glu 4247	-	expression tag
Ala 4248	-	expression tag
Ala 4249	-	expression tag
Ala 4250	-	expression tag
Lys 4251	-	expression tag
Glu 4252	-	expression tag
Ala 4253	-	expression tag
Ala 4254	-	expression tag
Ala 4255	-	expression tag
Lys 4256	-	expression tag
Glu 4257	-	expression tag
Ala 4258	-	expression tag
Ala 4259	-	expression tag
Ala 4260	-	expression tag
Lys 4261	-	expression tag
Glu 4262	-	expression tag
Ala 4263	-	expression tag
Ala 4264	-	expression tag
Ala 4265	-	expression tag
Lys 4266	-	expression tag
Ala 4267	-	expression tag
His 4268	-	expression tag
His 4269	-	expression tag
His 4270	-	expression tag
His 4271	-	expression tag
His 4272	-	expression tag
His 4273	-	expression tag
His 4274	-	expression tag
His 4275	-	expression tag
His 4276	-	expression tag
His 4277	-	expression tag

C, D: 24 - 575 (UniProt: P34664)

PDB	External Database	Details
-	Gly 204	deletion
-	Ala 205	deletion
-	Glu 206	deletion
-	Met 207	deletion
-	Ala 208	deletion
-	Ala 209	deletion
-	Leu 210	deletion
-	Leu 211	deletion
-	Arg 212	deletion
-	Asn 213	deletion
-	Met 214	deletion
-	Gly 215	deletion
-	Pro 216	deletion
-	Asp 217	deletion
-	Ser 218	deletion
-	Val 219	deletion
-	Glu 220	deletion
-	Asp 221	deletion
-	Ile 222	deletion
-	Arg 223	deletion
-	Arg 224	deletion
-	Phe 225	deletion
-	Thr 226	deletion
Ser 576	-	expression tag
Ala 577	-	expression tag
Trp 578	-	expression tag
Ser 579	-	expression tag
His 580	-	expression tag
Pro 581	-	expression tag
Gln 582	-	expression tag
Phe 583	-	expression tag
Glu 584	-	expression tag
Lys 585	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	1102315.2
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	1102446.0

*Water molecules are not included.