9QX0

Cryo-EM structure of the human UBR4/KCMF1/CALM1 complex (C-term focused refinement)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (D, C)	E3 ubiquitin-protein ligase KCMF1	polymer	391	43192.6	2	UniProt (Q9P0J7) Pfam (PF00569) Pfam (PF05605)	Homo sapiens (human)	FGF-induced in gastric cancer,Potassium channel modulatory factor,PCMF,ZZ-type zinc finger-containing protein 1
2	B (F)	Calmodulin-1	polymer	158	17980.7	1	UniProt (P0DP23) Pfam (PF13499)	Homo sapiens (human)
3	C, E (A, B)	E3 ubiquitin-protein ligase UBR4	polymer	5193	575844.0	2	UniProt (Q5T4S7) Pfam (PF19423) Pfam (PF02207) Pfam (PF13764)	Homo sapiens (human)	600 kDa retinoblastoma protein-associated factor,p600,N-recognin-4,Retinoblastoma-associated factor of 600 kDa,RBAF600

Sequence modifications

D, C: 1 - 381 (UniProt: Q9P0J7)

PDB	External Database	Details
Ser 382	-	expression tag
Ala 383	-	expression tag
Trp 384	-	expression tag
Ser 385	-	expression tag
His 386	-	expression tag
Pro 387	-	expression tag
Gln 388	-	expression tag
Phe 389	-	expression tag
Glu 390	-	expression tag
Lys 391	-	expression tag

F: 1 - 149 (UniProt: P0DP23)

PDB	External Database	Details
Met -8	-	initiating methionine
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag

A, B: 1 - 5183 (UniProt: Q5T4S7)

PDB	External Database	Details
His 5184	-	expression tag
His 5185	-	expression tag
His 5186	-	expression tag
His 5187	-	expression tag
His 5188	-	expression tag
His 5189	-	expression tag
His 5190	-	expression tag
His 5191	-	expression tag
His 5192	-	expression tag
His 5193	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	1256054.0
All*	Total formula weight	1256054.0

*Water molecules are not included.