9QVM
Cryo-EM reconstruction of the NEDD1 anchor protein and CDK5RAP2 bound to the gamma-tubulin ring complex
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, AA, B, BA, C... (D, F, H, r, s...) | Gamma-tubulin complex component 3 | polymer | 907 | 103710.1 | 10 | UniProt (Q96CW5) Pfam (PF17681) Pfam (PF04130) | Homo sapiens (human) | hGCP3,Gamma-ring complex protein 104 kDa,hGrip104,Spindle pole body protein Spc98 homolog,hSpc98 |
| 2 | D, E, F, G, H... (O, P, Q, R, S...) | Mitotic-spindle organizing protein 1 | polymer | 82 | 8485.7 | 7 | UniProt (Q08AG7) Pfam (PF12554) | Homo sapiens (human) | Mitotic-spindle organizing protein associated with a ring of gamma-tubulin 1 |
| 3 | JA, K, KA, L (W, X, V, Y) | Protein NEDD1 | polymer | 660 | 72051.0 | 4 | UniProt (Q8NHV4) Pfam (PF00400) | Homo sapiens (human) | Neural precursor cell expressed developmentally down-regulated protein 1,NEDD-1 |
| 4 | M (Z) | Actin, cytoplasmic 1, N-terminally processed | polymer | 375 | 41782.7 | 1 | UniProt (P60709) Pfam (PF00022) | Homo sapiens (human) | |
| 5 | IA, N, O, P, Q... (a, b, c, e, f...) | Tubulin gamma-1 chain | polymer | 457 | 52022.6 | 14 | UniProt (P23258) Pfam (PF00091) Pfam (PF03953) | Homo sapiens (human) | Gamma-1-tubulin,Gamma-tubulin complex component 1,GCP-1 |
| 6 | GA, RA (K, I) | Gamma-tubulin complex component 4 | polymer | 667 | 76180.0 | 2 | UniProt (Q9UGJ1) Pfam (PF17681) Pfam (PF04130) | Homo sapiens (human) | hGCP4,Gamma-ring complex protein 76 kDa,hGrip76 |
| 7 | LA, NA, PA, QA, SA (G, C, A, E, M) | Isoform 3 of Gamma-tubulin complex component 2 | polymer | 930 | 105765.7 | 5 | UniProt (Q9BSJ2) Pfam (PF17681) Pfam (PF04130) | Homo sapiens (human) | GCP-2,hGCP2,Gamma-ring complex protein 103 kDa,h103p,hGrip103,Spindle pole body protein Spc97 homolog,hSpc97 |
| 8 | MA (L) | TUBGCP6 protein | polymer | 1811 | 199732.5 | 1 | UniProt (B2RWN4) Pfam (PF17681) Pfam (PF04130) Pfam (PF19340) UniProt (by SIFTS) (Q96RT7) | Homo sapiens (human) | |
| 9 | OA (J) | Gamma-tubulin complex component 5 | polymer | 1024 | 118467.5 | 1 | UniProt (Q96RT8) Pfam (PF17681) Pfam (PF04130) | Homo sapiens (human) | GCP-5 |
| 10 | TA (p) | Mitotic-spindle organizing protein 2B | polymer | 158 | 16246.6 | 1 | UniProt (Q6NZ67) Pfam (PF12926) | Homo sapiens (human) | Mitotic-spindle organizing protein associated with a ring of gamma-tubulin 2B |
| 11 | UA, VA (x, w) | CDK5 regulatory subunit-associated protein 2 | polymer | 1663 | 189905.8 | 2 | UniProt (A0A0A0MRG9) Pfam (PF07989) | Homo sapiens (human) |
Sequence modifications
a, b, c, e, f, g, h, i, j, k, l, m, n, d: 1 - 451 (UniProt: P23258)
| PDB | External Database | Details |
|---|---|---|
| Glu 452 | - | expression tag |
| Asn 453 | - | expression tag |
| Leu 454 | - | expression tag |
| Tyr 455 | - | expression tag |
| Phe 456 | - | expression tag |
| Gln 457 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 48 |
| Total formula weight | 3550251.3 | |
| All* | Total formula weight | 3550251.3 |
