9QVA

Polyester Hydrolase Leipzig 7 (PHL7) variant R2M2-P155G-A186S

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Polyester Hydrolase Leipzig 7 (PHL-7), catalysis-deficient S131A mutant	polymer	267	28844.1	2	UniProt (A0AA82WPD4)	compost metagenome
2	C, D (A, B)	CITRIC ACID	non-polymer		192.1	2	Chemie (CIT) PubChem (311)
3	E, F (A, B)	water	water		18.0	698	Chemie (HOH)

Sequence modifications

A, B: 1 - 262 (UniProt: A0AA82WPD4)

PDB	External Database	Details
Gln 6	Glu 6	engineered mutation
Asp 34	Gln 34	engineered mutation
Ser 36	Asp 36	engineered mutation
Ser 68	Glu 68	engineered mutation
Ser 91	Thr 91	engineered mutation
Leu 104	Gln 104	engineered mutation
Tyr 109	His 109	engineered mutation
Thr 111	Arg 111	engineered mutation
Asp 113	Asn 113	engineered mutation
Thr 115	Val 115	engineered mutation
Asp 118	Asn 118	engineered mutation
Ser 131	Ala 131	engineered mutation
Pro 145	Thr 145	engineered mutation
Lys 148	Glu 148	engineered mutation
Asp 161	Asn 161	engineered mutation
Val 167	Thr 167	engineered mutation
Ile 171	Val 171	engineered mutation
Ser 196	Asp 196	engineered mutation
Pro 198	Asp 198	engineered mutation
Asp 208	Ser 208	engineered mutation
Asn 216	Asp 216	engineered mutation
Thr 235	Leu 235	engineered mutation
Pro 248	Phe 248	engineered mutation
His 263	-	expression tag
His 264	-	expression tag
His 265	-	expression tag
His 266	-	expression tag
His 267	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57688.2
Non-Polymers*	Number of molecules	2
	Total formula weight	384.2
All*	Total formula weight	58072.5

*Water molecules are not included.