9QTY
Cryo-EM structure of the binary MEGF8-MOSMO complex with nanobody 270
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Modulator of smoothened protein | polymer | 211 | 22594.2 | 1 | UniProt (Q8NHV5) Pfam (PF18800) | Homo sapiens (human) | Attenuator of hedgehog |
| 2 | B (C) | Nanobody 270 | polymer | 129 | 14378.9 | 1 | Lama glama | ||
| 3 | C (B) | Isoform 2 of Multiple epidermal growth factor-like domains protein 8 | polymer | 344 | 37349.2 | 1 | UniProt (Q7Z7M0) | Homo sapiens (human) | Multiple EGF-like domains protein 8,Epidermal growth factor-like protein 4,EGF-like protein 4 |
| 4 | D (A) | PALMITIC ACID | non-polymer | 256.4 | 1 | Chemie (PLM) PubChem (985) | Homo sapiens (human) | Attenuator of hedgehog | |
| 5 | E (B) | (1~{S},2~{S},4~{S},5'~{R},6~{R},7~{S},8~{R},9~{S},12~{S},13~{R},16~{S})-16-methoxy-5',7,9,13-tetramethyl-spiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icos-18-ene-6,2'-oxane] | non-polymer | 428.6 | 1 | Chemie (A1JAX) | |||
| 6 | F, G, H (A, C, B) | water | water | 18.0 | 10 | Chemie (HOH) |
Sequence modifications
A: 1 - 167 (UniProt: Q8NHV5)
B: 2312 - 2645 (UniProt: Q7Z7M0)
| PDB | External Database | Details |
|---|---|---|
| Gly 168 | - | linker |
| Thr 169 | - | linker |
| Leu 170 | - | expression tag |
| Glu 171 | - | expression tag |
| Val 172 | - | expression tag |
| Leu 173 | - | expression tag |
| Phe 174 | - | expression tag |
| Gln 175 | - | expression tag |
| Gly 176 | - | expression tag |
| Pro 177 | - | expression tag |
| Gly 178 | - | linker |
| Gly 179 | - | linker |
| Ser 180 | - | linker |
| Gly 181 | - | linker |
| Ser 182 | - | expression tag |
| Ala 183 | - | expression tag |
| Trp 184 | - | expression tag |
| Ser 185 | - | expression tag |
| His 186 | - | expression tag |
| Pro 187 | - | expression tag |
| Gln 188 | - | expression tag |
| Phe 189 | - | expression tag |
| Glu 190 | - | expression tag |
| Lys 191 | - | expression tag |
| Gly 192 | - | expression tag |
| Gly 193 | - | expression tag |
| Gly 194 | - | expression tag |
| Ser 195 | - | expression tag |
| Gly 196 | - | expression tag |
| Gly 197 | - | expression tag |
| Gly 198 | - | expression tag |
| Ser 199 | - | expression tag |
| Gly 200 | - | expression tag |
| Gly 201 | - | expression tag |
| Ser 202 | - | expression tag |
| Ala 203 | - | expression tag |
| Trp 204 | - | expression tag |
| Ser 205 | - | expression tag |
| His 206 | - | expression tag |
| Pro 207 | - | expression tag |
| Gln 208 | - | expression tag |
| Phe 209 | - | expression tag |
| Glu 210 | - | expression tag |
| Lys 211 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 2646 | - | expression tag |
| Thr 2647 | - | expression tag |
| Glu 2648 | - | expression tag |
| Thr 2649 | - | expression tag |
| Ser 2650 | - | expression tag |
| Gln 2651 | - | expression tag |
| Val 2652 | - | expression tag |
| Ala 2653 | - | expression tag |
| Pro 2654 | - | expression tag |
| Ala 2655 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 74322.3 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 685.1 | |
| All* | Total formula weight | 75007.4 |
