9QPT

Single particle cryo-EM structure of MdtF V610F with bound Rhodamine 6G

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Multidrug resistance protein MdtF	polymer	1055	113807.9	3	UniProt (P37637) Pfam (PF00873)	Escherichia coli K-12
2	AA, BA, CA, D, KA... (B, A, C)	DODECANE	non-polymer		170.3	12	Chemie (D12)
3	E, EA, F, FA, G... (A, C, B)	PHOSPHATIDYLETHANOLAMINE	non-polymer		734.0	23	Chemie (PTY)
4	DA (C)	RHODAMINE 6G	non-polymer		443.6	1	Chemie (RHQ)

Sequence modifications

A, B, C: 1 - 1037 (UniProt: P37637)

PDB	External Database	Details
Phe 610	Val 610	engineered mutation
Ala 1038	-	expression tag
Ser 1039	-	expression tag
Gly 1040	-	expression tag
Glu 1041	-	expression tag
Asn 1042	-	expression tag
Leu 1043	-	expression tag
Tyr 1044	-	expression tag
Phe 1045	-	expression tag
Gln 1046	-	expression tag
Ser 1047	-	expression tag
Leu 1048	-	expression tag
Glu 1049	-	expression tag
His 1050	-	expression tag
His 1051	-	expression tag
His 1052	-	expression tag
His 1053	-	expression tag
His 1054	-	expression tag
His 1055	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	341423.7
Non-Polymers*	Number of molecules	36
	Total formula weight	19370.5
All*	Total formula weight	360794.2

*Water molecules are not included.