9QNR

Cryo-EM structure of TTYH3 in GDN after incubation with ApoE, map2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Protein tweety homolog 3	polymer	588	64776.4	2	UniProt (Q9C0H2) Pfam (PF04906)	Homo sapiens (human)	hTTY3,Volume-regulated anion channel subunit TTYH3
2	C, D (C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
3	E, F, G, H (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)

Sequence modifications

A, B: 2 - 523 (UniProt: Q9C0H2)

PDB	External Database	Details
Met 0	-	initiating methionine
Ser 1	-	expression tag
Ala 524	-	expression tag
Leu 525	-	expression tag
Glu 526	-	expression tag
Val 527	-	expression tag
Leu 528	-	expression tag
Phe 529	-	expression tag
Gln 530	-	expression tag
Gly 531	-	expression tag
Pro 532	-	expression tag
Gln 533	-	expression tag
Gly 534	-	expression tag
Thr 535	-	expression tag
Glu 536	-	expression tag
Gln 537	-	expression tag
Lys 538	-	expression tag
Leu 539	-	expression tag
Ile 540	-	expression tag
Ser 541	-	expression tag
Glu 542	-	expression tag
Glu 543	-	expression tag
Asp 544	-	expression tag
Leu 545	-	expression tag
Arg 546	-	expression tag
Gly 547	-	expression tag
Ala 548	-	expression tag
Ser 549	-	expression tag
Met 550	-	expression tag
Asp 551	-	expression tag
Glu 552	-	expression tag
Lys 553	-	expression tag
Thr 554	-	expression tag
Thr 555	-	expression tag
Gly 556	-	expression tag
Trp 557	-	expression tag
Arg 558	-	expression tag
Gly 559	-	expression tag
Gly 560	-	expression tag
His 561	-	expression tag
Val 562	-	expression tag
Val 563	-	expression tag
Glu 564	-	expression tag
Gly 565	-	expression tag
Leu 566	-	expression tag
Ala 567	-	expression tag
Gly 568	-	expression tag
Glu 569	-	expression tag
Leu 570	-	expression tag
Glu 571	-	expression tag
Gln 572	-	expression tag
Leu 573	-	expression tag
Arg 574	-	expression tag
Ala 575	-	expression tag
Arg 576	-	expression tag
Leu 577	-	expression tag
Glu 578	-	expression tag
His 579	-	expression tag
His 580	-	expression tag
Pro 581	-	expression tag
Gln 582	-	expression tag
Gly 583	-	expression tag
Gln 584	-	expression tag
Arg 585	-	expression tag
Glu 586	-	expression tag
Pro 587	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	129552.8
Branched	Number of molecules	2
	Total formula weight	848.8
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	131286.4

*Water molecules are not included.