9QGX
SciA(H42A) endonuclease from Escherichia phage T5 in complex with 10 bp DNA (GCGC sequence 1)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Nicking endonuclease | polymer | 148 | 17235.7 | 1 | UniProt (Q6QGD4) | Escherichia phage T5 | |
| 2 | B (B) | DNA (5'-D(*C*CP*AP*GP*GP*CP*GP*CP*AP*G)-3') | polymer | 10 | 3055.0 | 1 | Escherichia phage T5 | ||
| 3 | C (C) | DNA (5'-D(*C*TP*GP*CP*GP*CP*CP*TP*GP*G)-3') | polymer | 10 | 3037.0 | 1 | Escherichia phage T5 | ||
| 4 | D (A) | IMIDAZOLE | non-polymer | 69.1 | 1 | Chemie (IMD) | |||
| 5 | E (A) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) PubChem (175) | |||
| 6 | F, G (A) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 7 | H, I, J (A, B, C) | water | water | 18.0 | 27 | Chemie (HOH) |
Sequence modifications
A: 1 - 145 (UniProt: Q6QGD4)
| PDB | External Database | Details |
|---|---|---|
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Ala 42 | His 42 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 23327.7 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 258.9 | |
| All* | Total formula weight | 23586.7 |
