9QGC
Crystal structure of an NADH-accepting ene reductase variant NostocER1-L1,5 mutant Q204K
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (D) | All1865 protein | polymer | 375 | 40725.4 | 1 | UniProt (Q8YVV8) Pfam (PF00724) | Nostoc sp. PCC 7120 = FACHB-418 | |
| 2 | B (D) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 1 | Chemie (FMN) | |||
| 3 | C, D, F, G, H... (D) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 6 | Chemie (EDO) | |||
| 4 | E (D) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
| 5 | J (D) | water | water | 18.0 | 467 | Chemie (HOH) |
Sequence modifications
D: 12 - 375 (UniProt: Q8YVV8)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Gly 2 | - | expression tag |
| His 3 | - | expression tag |
| His 4 | - | expression tag |
| His 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| His 8 | - | expression tag |
| Gly 9 | - | expression tag |
| Ser 10 | - | expression tag |
| Gly 11 | - | expression tag |
| Ser 40 | Gln 67 | conflict |
| Lys 204 | Gln 231 | engineered mutation |
| Leu 243 | Ser 270 | conflict |
| Thr 244 | Gly 271 | conflict |
| Leu 246 | Phe 273 | conflict |
| Gly 248 | Asp 275 | conflict |
| Cys 249 | Ile 276 | conflict |
| Val 250 | Arg 277 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 40725.4 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 887.8 | |
| All* | Total formula weight | 41613.2 |
