9QGB
Crystal structure of an NADH-accepting ene reductase variant NostocER1-L1,5 (engineered Loop Swap from Achromobacter sp. JA81)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | All1865 protein | polymer | 375 | 40724.3 | 2 | UniProt (Q8YVV8) | Nostoc sp. PCC 7120 = FACHB-418 | |
| 2 | C, E, G, H, I... (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 18 | Chemie (EDO) | |||
| 3 | D, M (A, B) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 2 | Chemie (FMN) | |||
| 4 | F, P (A, B) | ACETATE ION | non-polymer | 59.0 | 2 | Chemie (ACT) | |||
| 5 | Y (B) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 6 | Z (B) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 7 | AA, BA (A, B) | water | water | 18.0 | 1151 | Chemie (HOH) |
Sequence modifications
A, B: 12 - 375 (UniProt: Q8YVV8)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Gly 2 | - | expression tag |
| His 3 | - | expression tag |
| His 4 | - | expression tag |
| His 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| His 8 | - | expression tag |
| Gly 9 | - | expression tag |
| Ser 10 | - | expression tag |
| Gly 11 | - | expression tag |
| Ser 40 | Gln 67 | conflict |
| Leu 243 | Ser 270 | conflict |
| Thr 244 | Gly 271 | conflict |
| Leu 246 | Phe 273 | conflict |
| Gly 248 | Asp 275 | conflict |
| Cys 249 | Ile 276 | conflict |
| Val 250 | Arg 277 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 81448.7 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 2206.4 | |
| All* | Total formula weight | 83655.1 |
